PA5-90801

antibody from Invitrogen Antibodies

Targeting: B3GNT4 B3GN-T4, beta3Gn-T4

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-90801

Provider

Invitrogen Antibodies

Product name

B3GNT4 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: APPAQLLAYE SREFDDILQW DFTEDFFNLT LKELHLQRWV VAACPQAHFM LKGDDDVFVH VPNVLEFLDG WDPAQDLLVG DVIRQALPNR NTKVKYFIPP SMYRATHYPP YAGGGGYVMS RATVRRLQAI MEDAELFPID D; Positive Samples: Rat brain, Rat intestine; Cellular Location: Golgi apparatus membrane, Single-pass type II membrane protein

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1.38 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of B3GNT4 in extracts of various cell lines using B3GNT4 Polyclonal Antibody (Product # PA5-90801) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of B3GNT4 was performed in C6 cells using B3GNT4 Polyclonal Antibody (Product # PA5-90801) at a dilution of 1:100. Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of B3GNT4 was performed in HeLa cells using B3GNT4 Polyclonal Antibody (Product # PA5-90801) at a dilution of 1:100. Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of B3GNT4 was performed in L929 cells using B3GNT4 Polyclonal Antibody (Product # PA5-90801) at a dilution of 1:100. Blue: DAPI for nuclear staining.