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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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- Product number
- GTX104040 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX104040, RRID:AB_11170089
- Product name
- Pyruvate Dehydrogenase E1 beta subunit antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
Submitted references Protein kinase B (PKB/AKT1) formed signaling complexes with mitochondrial proteins and prevented glycolytic energy dysfunction in cultured cardiomyocytes during ischemia-reperfusion injury.
Deng W, Leu HB, Chen Y, Chen YH, Epperson CM, Juang C, Wang PH
Endocrinology 2014 May;155(5):1618-28
Endocrinology 2014 May;155(5):1618-28
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Supportive validation
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- Experimental details
- Pyruvate Dehydrogenase E1 beta subunit antibody detects PDHB protein by Western blot analysis.A. 50 ?g rat heart lysate/extract10 % SDS-PAGEPyruvate Dehydrogenase E1 beta subunit antibody (GTX104040) dilution: 1:5000
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- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: A549 B: H1299 C: HCT116 10% SDS PAGE GTX104040 diluted at 1:1000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (50 ug of whole cell lysate) A: mouse heart 10% SDS PAGE GTX104040 diluted at 1:10000
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Pyruvate Dehydrogenase E1 beta subunit antibody detects Pyruvate Dehydrogenase E1 beta subunit protein at mitochondria by immunofluorescent analysis.Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.Green: Pyruvate Dehydrogenase E1 beta subunit protein stained by Pyruvate Dehydrogenase E1 beta subunit antibody (GTX104040) diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar = 10 £gm.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Pyruvate Dehydrogenase E1 beta subunit antibody detects Pyruvate Dehydrogenase E1 beta subunit protein at cytoplasm in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. Pyruvate Dehydrogenase E1 beta subunit antibody (GTX104040) diluted at 1:500.
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- GeneTex (provider)
- Main image
- Experimental details
- Pyruvate Dehydrogenase E1 beta subunit antibody detects Pyruvate Dehydrogenase E1 beta subunit protein at cytoplasm in mouse intestine by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. Pyruvate Dehydrogenase E1 beta subunit antibody (GTX104040) diluted at 1:500.
