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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [5]
- Immunocytochemistry [1]
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- Product number
- PA5-22220 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SESN3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: HeLa, Raw264.7, C2C12, Rat2.
- Concentration
- 1.36 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SESN3 using A) 30 µg Raw264.7 whole cell lysate and B) 30 µg C2C12 whole cell lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a SESN3 polyclonal antibody (Product # PA5-22220) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SESN3 using 30 µg of HeLa lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a SESN3 polyclonal antibody (Product # PA5-22220) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of SESN3 was performed by separating 30 µg of various whole cell extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a SESN3 Polyclonal Antibody (Product # PA5-22220) at a dilution of 1:500 and a HRP-conjugated anti-rabbit IgG secondary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using SESN3 Polyclonal Antibody (Product # PA5-22220). Various tissue extracts (50 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with SESN3 Polyclonal Antibody (Product # PA5-22220) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- SESN3 Polyclonal Antibody detects SESN3 protein by western blot analysis. A. 30 µg Rat2 whole cell lysate/extract.7.5% SDS-PAGE. SESN3 Polyclonal Antibody (Product # PA5-22220) dilution: 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of SESN3 in paraformaldehyde-fixed A549 cells using a SESN3 polyclonal antibody (Product # PA5-22220) at a 1:200 dilution.
