NBP2-20415
antibody from Novus Biologicals
Targeting: SMARCC1
BAF155, CRACC1, Rsc8, SRG3
Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [4]
- Immunoprecipitation [1]
- Immunohistochemistry [6]
- Chromatin Immunoprecipitation [1]
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Validation data
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- Product number
- NBP2-20415 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal SMARCC1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Initiating Events in Direct Cardiomyocyte Reprogramming.
Sauls K, Greco TM, Wang L, Zou M, Villasmil M, Qian L, Cristea IM, Conlon FL
Cell reports 2018 Feb 13;22(7):1913-1922
Cell reports 2018 Feb 13;22(7):1913-1922
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SMARCC1 Antibody [NBP2-20415] - Sample (30 ug of whole cell lysate) A: NT2D1 B: IMR32 C: U87-MG 5% SDS PAGE gel, diluted at 1:5000.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SMARCC1 Antibody [NBP2-20415] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with SMARCC1 antibody [C2C3], C-term diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SMARCC1 Antibody [NBP2-20415] - Wild-type (WT) and SMARCC1 knockout (KO) HeLa cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with SMARCC1 antibody diluted at 1:5000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SMARCC1 Antibody [NBP2-20415] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with SMARCC1 antibody diluted at 1:2000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: SMARCC1 Antibody [NBP2-20415] - SMARCC1 antibody immunoprecipitates SMARCC1 protein in IP experiments. IP Sample: 293T whole cell lysate/extract A : 30 ug whole cell lysate/extract of SMARCC1 protein expressing 293T cells B : Control with 2.5 ug of pre-immune rabbit IgG C : Immunoprecipitation of SMARCC1 by 2.5 ug of SMARCC1 antibody 5% SDS-PAGE The mmunoprecipitated SMARCC1 protein was detected by SMARCC1 antibody diluted at 1:1000. EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SMARCC1 Antibody [NBP2-20415] - Immunohistochemical analysis of paraffin-embedded C2C12 xenograft, using antibody at 1:500 dilution.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SMARCC1 Antibody [NBP2-20415] - Immunohistochemical analysis of paraffin-embedded RT2 xenograft, using antibody at 1:500 dilution.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SMARCC1 Antibody [NBP2-20415] - Immunohistochemical analysis of paraffin-embedded BT483 xenograft, using antibody at 1:500 dilution.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SMARCC1 Antibody [NBP2-20415] - Paraffin-embedded mouse prostate dilution: 1:500.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SMARCC1 Antibody [NBP2-20415] - Paraffin-embedded rat hind brain dilution: 1:500.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SMARCC1 Antibody [NBP2-20415] - Paraffin-embedded mouse duodenum dilution: 1:50
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Chromatin Immunoprecipitation: SMARCC1 Antibody [NBP2-20415] - Cross-linked ChIP was performed with MF-7 chromatin extract treated with B-estradiol (10 nM for 45 min) and 5 ug of either control rabbit IgG or anti-SMARCC1 antibody. The precipitated DNA was detected by PCR with primer set targeting to ESR1 or Sat2.