MA5-34657

antibody from Invitrogen Antibodies

Targeting: SMARCC1 BAF155, CRACC1, Rsc8, SRG3

Immunoprecipitation

Antibody data

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Validation data

Product number: MA5-34657 - Provider product page
Provider: Invitrogen Antibodies
Product name: SMARCC1 Recombinant Rabbit Monoclonal Antibody (JB43-43)
Antibody type: Monoclonal
Antigen: Recombinant full-length protein
Description: Positive Control: Mouse thymus tissue, HL-60, 293T, mouse testis tissue, K562.
Reactivity: Human, Mouse
Host: Rabbit
Isotype: IgG
Antibody clone number: JB43-43
Vial size: 100 µL
Concentration: 1 mg/mL
Storage: -20° C, Avoid Freeze/Thaw Cycles, store in dark

SMARCC1 protein structure - MA5-34657 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of SMARCC1 in Positive control: Line 1: Mouse thymus tissueLine 2: HL-60. Samples were incubated with SMARCC1 monoclonal antibody (Product # MA5-34657), at a dilution of 1:500.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot was performed using Anti-SMARCC1 Recombinant Rabbit Monoclonal Antibody (JB43-43) (Product # MA5-34657) and a 155 kDa band corresponding to SMARCC1 was observed across cell lines. Whole cell extracts (30 µg lysate) of HeLa (Lane 1), MCF7 (Lane 2), MIA PaCa-2 (Lane 3), LNCaP (Lane 4), PC-3 (Lane 5), DU 145 (Lane 6), MCF7 (Lane 7), Neuro-2a (Lane 8) were electrophoresed using NuPAGE™ 3-8% Tris-Acetate Protein Gel (Product # EA0378BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23002) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:20000 dilution) using the iBright™ FL1500 Imaging System (Product # A44115). Chemiluminescent detection was performed using SuperSignal™ West Pico PLUS Chemiluminescent Substrate (Product # 34580).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Knockdown of SMARCC1 was achieved by transfecting HeLa with SMARCC1 specific siRNAs (Silencer® select Product # s13145, s13147). Western blot analysis (Fig. a) was performed using Whole cell extracts from the SMARCC1 knockdown cells (lane 3), non-targeting scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with SMARCC1 Recombinant Rabbit Monoclonal Antibody (JB43-43) (Product # MA5-34657, 1:1000 dilution ) and Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:20000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to SMARCC1.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence analysis of SMARCC1 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with SMARCC1 Recombinant Rabbit Monoclonal Antibody (JB43-43) (Product # MA5-34657) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790), (1:2000 dilution), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b:Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300 dilution). Panel d represents the merged image showing nuclear and cytosolic localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X with oil immersion magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Knockdown of SMARCC1 was achieved by transfecting HeLa cells with SMARCC1 specific siRNA (Silencer® select Product # s13145, s13147). Immunofluorescence analysis was performed on untransfected HeLa cells (panel a,d), transfected with non-specific scrambled siRNA (panels b,e) and transfected with SMARCC1 specific siRNA (panel c,f). Cells were fixed, permeabilized, and labelled with SMARCC1 Recombinant Rabbit Monoclonal Antibody (JB43-43) (Product # MA5-34657, 1:100 dilution) followed by Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790), (1:2000 dilution). Nuclei (blue) were stained using ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962), and Rhodamine Phalloidin (Product # R415, 1:300 dilution) was used for cytoskeletal F-actin (Red) staining. Decrease in intensity of specific signal was observed upon siRNA mediated knockdown (panel c,f) confirming specificity of the antibody to SMARCC1 (Green). The Images were captured at 60x oil immersion magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of SMARCC1 in 293T cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with SMARCC1 monoclonal antibody (Product # MA5-34657), followed by DAPI (blue).