17653-1-AP

antibody from Invitrogen Antibodies

Targeting: GTF3C4 KAT12, TFIIIC90

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

17653-1-AP

Provider

Invitrogen Antibodies

Product name

GTF3C4 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: GSVRTHGIA VSPCGAYLAI ITTEGMINGL HPVNKNYQVQ FVTLKTFEEA AAQLLESSVQ NLFKQVDLID LVRWKILKDK HIPQFLQEAL EKKIESSGVT YFWRFKLFLL RILYQSMQKT PSEALWKPTH EDSKILLVDS PGMGNADDEQ QEEGTSSKQV VKQGLQERSK EGDVEEPTDD SLPTTGDAGG REPMEEKLLE IQGKIEAVEM HLTREHMKRV LGEVYLHTWI TENTSIPTRG LCNFLMSDEE YDDRTARVLI GHISKKMNKQ TFPEHCSLCK EILPFTDRKQ AVCSNGHIWL RCFLTYQSCQ SLIYRRCLLH DSIARHPAPE DPDWIKRLLQ SPCPFCDSPV F (473-822 aa encoded by BC094774)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.37 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 17653-1-AP (GTF3C4 antibody) at dilution of 1:1600 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 17653-1-AP (GTF3C4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ( 10% Formaldehyde ) fixed HeLa cells using 17653-1-AP (GTF3C4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 17653-1-AP ( GTF3C4 Antibody) at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 17653-1-AP ( GTF3C4 Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-GTF3C4 (IP:17653-1-AP, 3ug; Detection:17653-1-AP 1:800) with HeLa cells lysate 875ug.