PA5-30863

antibody from Invitrogen Antibodies

Targeting: HIP1R FLJ14000, HIP12, HIP3, ILWEQ, KIAA0655

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-30863

Provider

Invitrogen Antibodies

Product name

HIP1R Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: MCF-7.

Concentration

1 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using HIP1R Polyclonal Antibody (Product # PA5-30863). Sample (30 µg of whole cell lysate). Lane A: MCF-7 . 7.5% SDS PAGE. HIP1R Polyclonal Antibody (Product # PA5-30863) diluted at 1:1,000.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HIP1 Related antibody [N1N2], N-term detects HIP1R protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HIP1R protein stained by HIP1 Related antibody [N1N2], N-term (Product # PA5-30863) diluted at 1:500. Blue: Hoechst 33343 staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HIP1 Related antibody [N1N2], N-term detects HIP1R protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HIP1R protein stained by HIP1 Related antibody [N1N2], N-term (Product # PA5-30863) diluted at 1:500. Blue: Hoechst 33343 staining.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Paraffin) analysis of HIP1R was performed in paraffin-embedded rat brain tissue using HIP1R Polyclonal Antibody (Product # PA5-30863) at a dilution of 1:500.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using HIP1 Related (Product # PA5-30863) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.