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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- PAB2802 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB2802, RRID:AB_1580946
- Product name
- SPHK2 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of SPHK2.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Sphingosine 1-phosphate modulates spinal nociceptive processing.
Coste O, Brenneis C, Linke B, Pierre S, Maeurer C, Becker W, Schmidt H, Gao W, Geisslinger G, Scholich K
The Journal of biological chemistry 2008 Nov 21;283(47):32442-51
The Journal of biological chemistry 2008 Nov 21;283(47):32442-51
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- The SPHK2 polyclonal antibody (Cat # PAB2802) is used in Western blot (Lane 1) to detect c-myc-tagged SPHK2 in transfected 293 cell lysate (ac-myc antibody is used as control in Lane 2) . Data is kindly provided by Dr. J. Van Brocklyn from the Ohio State University (Columbus, OH) .
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with SPHK2 polyclonal antibody (Cat # PAB2802) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. HC = hepatocarcinoma.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
