H00004698-M02

antibody from Abnova Corporation

Targeting: NDUFA5 B13, CI-13kB, CI-13KD-B, NUFM, UQOR13

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

H00004698-M02

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00004698-M02, RRID:AB_10616925

Product name

NDUFA5 monoclonal antibody (M02), clone 4A2

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full-length recombinant NDUFA5.

Antigen sequence

MAGVLKKTTGLVGLAVCNTPHERLRILYTKILDVL
EEIPKNAAYRKYTEQITNEKLAMVKAEPDVKKLED
QLQGGQLEEVILQAEHELNLARKMREWKLWEPLVE
EPPADQWKWPI


Isotype

IgG

Antibody clone number

4A2

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of NDUFA5 expression in transfected 293T cell line by NDUFA5 monoclonal antibody (M02), clone 4A2.Lane 1: NDUFA5 transfected lysate(13.5 KDa).Lane 2: Non-transfected lysate.

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

NDUFA5 monoclonal antibody (M02), clone 4A2. Western Blot analysis of NDUFA5 expression in human kidney.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged NDUFA5 is 10 ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunoperoxidase of monoclonal antibody to NDUFA5 on formalin-fixed paraffin-embedded human heart. [antibody concentration 3 ug/ml]

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Protocol

Protocol