Antibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- H00051703-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00051703-M01, RRID:AB_538690
- Product name
- Mouse Monoclonal ACSL5 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. ACSL5 - acyl-CoA synthetase long-chain family member 5
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Modulating effects of acyl-CoA synthetase 5-derived mitochondrial Wnt2B palmitoylation on intestinal Wnt activity.
TP53 status regulates ACSL5-induced expression of mitochondrial mortalin in enterocytes and colorectal adenocarcinomas.
Gene expression profiling in sinonasal adenocarcinoma.
Promotion of glioma cell survival by acyl-CoA synthetase 5 under extracellular acidosis conditions.
Klaus C, Schneider U, Hedberg C, Schütz AK, Bernhagen J, Waldmann H, Gassler N, Kaemmerer E
World journal of gastroenterology 2014 Oct 28;20(40):14855-64
World journal of gastroenterology 2014 Oct 28;20(40):14855-64
TP53 status regulates ACSL5-induced expression of mitochondrial mortalin in enterocytes and colorectal adenocarcinomas.
Klaus C, Kaemmerer E, Reinartz A, Schneider U, Plum P, Jeon MK, Hose J, Hartmann F, Schnölzer M, Wagner N, Kopitz J, Gassler N
Cell and tissue research 2014 Jul;357(1):267-78
Cell and tissue research 2014 Jul;357(1):267-78
Gene expression profiling in sinonasal adenocarcinoma.
Tripodi D, Quéméner S, Renaudin K, Ferron C, Malard O, Guisle-Marsollier I, Sébille-Rivain V, Verger C, Géraut C, Gratas-Rabbia-Ré C
BMC medical genomics 2009 Nov 10;2:65
BMC medical genomics 2009 Nov 10;2:65
Promotion of glioma cell survival by acyl-CoA synthetase 5 under extracellular acidosis conditions.
Mashima T, Sato S, Sugimoto Y, Tsuruo T, Seimiya H
Oncogene 2009 Jan 8;28(1):9-19
Oncogene 2009 Jan 8;28(1):9-19
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ACSL5 Antibody (5H8) [H00051703-M01] - Analysis of ACSL5 expression in transfected 293T cell line by ACSL5 monoclonal antibody (M01), clone 5H8.Lane 1: ACSL5 transfected lysate(82.3 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ACSL5 Antibody (5H8) [H00051703-M01] - ACSL5 monoclonal antibody (M01), clone 5H8 Analysis of ACSL5 expression in HepG2.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: ACSL5 Antibody (5H8) [H00051703-M01] - Detection limit for recombinant GST tagged ACSL5 is approximately 0.1ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: ACSL5 Antibody (5H8) [H00051703-M01] - Analysis of ACSL5 transfected lysate using anti-ACSL5 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with ACSL5 MaxPab rabbit polyclonal antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: ACSL5 Antibody (5H8) [H00051703-M01] - Analysis of monoclonal antibody to ACSL5 on formalin-fixed paraffin-embedded human colon. Antibody concentration 3 ug/ml.