H00000381-M02

antibody from Abnova Corporation

Targeting: ARF5

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

H00000381-M02

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00000381-M02, RRID:AB_1137091

Product name

ARF5 monoclonal antibody (M02), clone 1B6

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a partial recombinant ARF5.

Antigen sequence

YFQNTQGLIFVVDSNDRERVQESADELQKMLQEDE
LRDAVLLVFANKQDMPNAMPVSELTDKLGLQHLRS
RTWYVQATCATQGTGLYDGLDWLSHELSKR


Isotype

IgG

Antibody clone number

1B6

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of ARF5 expression in transfected 293T cell line by ARF5 monoclonal antibody (M02), clone 1B6.Lane 1: ARF5 transfected lysate(20.5 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged ARF5 is approximately 0.3ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol