AP43239PU-N

antibody from Acris Antibodies GmbH

Targeting: ATP6V0A2 a2, ATP6a2, ATP6N1D, J6B7, Stv1, TJ6, TJ6M, TJ6s, Vph1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AP43239PU-N

Provider

Acris Antibodies GmbH

Product name

anti ATP6V0A2

Antibody type

Polyclonal

Antigen

Synthetic peptide directed towards the N-terminal of human ATP6V0A2.

Reactivity

Human, Mouse, Rat, Bovine, Canine, Chicken/Avian, Zebrafish

Host

Rabbit

Vial size

50 µg

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Human Hela; . Application: Western blotting. Species+tissue/cell type: HeLa cells. How many ug'sof tissue/cell lysate run on the gel:1. 10 ug untransfected HeLa lysate2. 10 ug mATP6V0A2 (Partial) transfected HeLa lysate3. 10 ug mATP6V0A2-FLAG transfected HeLa lysate4. 10 ug mATP6V0A1-FLAG transfected HeLa lysate. Primary antibody dilution: 1:300. Secondary antibody: Anti-rabbit-HRP. Secondary antibody dilution: 1:1000; ATP6V0A2 antibody - N-terminal region (AP43239PU-N) in Human Hela cells using Western Blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Human HeLa; ATP6V0A2 antibody - N-terminal region (AP43239PU-N) validated by WB using HeLa cells at 1:300.; ATP6V0A2 antibody - N-terminal region (AP43239PU-N) in Human HeLa cells using Western Blot

Immunohistochemistry

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Human Hela; . Application: IHC/Immunofluorescence. Species+tissue/cell type:A. untransfected HeLa cellsB. mATP6V0A2-FLAG transfected HeLa cellsC. mATP6V0A2 (partial) transfected HeLa cells. Primary antibody dilution: 1:250. Secondary antibody: Anti-rabbit AlexaFluor 488. Secondary antibody dilution: 1:1000; ATP6V0A2 antibody - N-terminal region (AP43239PU-N) in Human Hela cells using Immunohistochemistry

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

human kidney; Anti-ATP6V0A2 antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.; ATP6V0A2 antibody - N-terminal region (AP43239PU-N) in human kidney cells using Immunohistochemistry

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Human small intestine; Anti-ATP6V0A2 antibody IHC staining of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.; ATP6V0A2 antibody - N-terminal region (AP43239PU-N) in Human small intestine cells using Immunohistochemistry

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Human kidney; Immunohistochemistry with Human kidney lysate tissue at an antibody concentration of 5.0ug/ml using anti-ATP6V0A2 antibody (AP43239PU-N); ATP6V0A2 antibody - N-terminal region (AP43239PU-N) in Human kidney cells using Immunohistochemistry