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LearnTA346466
antibody from OriGene
Targeting: ATP6V0A2
a2, ATP6a2, ATP6N1D, J6B7, Stv1, TJ6, TJ6M, TJ6s, Vph1
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [4]
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Validation data
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- Product number
- TA346466 - Provider product page
- Provider
- OriGene
- Product name
- Rabbit Polyclonal Anti-ATP6V0A2 Antibody
- Antibody type
- Polyclonal
- Description
- Rabbit Polyclonal Anti-ATP6V0A2 Antibody
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- ATP6V0A2
- Isotype
- IgG
- Antibody clone number
- NULL
- Vial size
- 100 µl
- Concentration
- NULL
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- ATP6V0A2 antibody - N-terminal region validated by WB using HeLa cells at 1: 300.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Application: Western blotting; Species+tissue/cell type: HeLa cells; How many ug'sof tissue/cell lysate run on the gel: 1. 10 ug untransfected HeLa lysate2. 10 ug mATP6V0A2 (Partial) transfected HeLa lysate3. 10 ug mATP6V0A2-FLAG transfected HeLa lysate4. 10 ug mATP6V0A1-FLAG transfected HeLa lysate; Primary antibody dilution: 1: 300; Secondary antibody: Anti-rabbit-HRP; Secondary antibody dilution: 1: 1000
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemistry with Human kidney lysate tissue at an antibody concentration of 5.0 ug/ml using anti-ATP6V0A2 antibody
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Application: IHC/Immunofluorescence; Species+tissue/cell type: A. untransfected HeLa cellsB. mATP6V0A2-FLAG transfected HeLa cellsC. mATP6V0A2 (partial) transfected HeLa cells; Primary antibody dilution: 1: 250; Secondary antibody: Anti-rabbit AlexaFluor 488; Secondary antibody dilution: 1: 1000
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-ATP6V0A2 antibody IHC staining of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-ATP6V0A2 antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
- Validation comment
- IHC
