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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Other assay [1]
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- Product number
- MA5-23149 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- STKLD1 Monoclonal Antibody (2B5)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- Peptide Sequence: MLGPGSNRRR PTQGERGPGS PGEPMEKYQV LYQLNPGALG VNLVVEEMET KVKHVIKQVE CMDDHYASQA LEELMPLLKL RHAHISVYQE LFITWNGEI
- Antibody clone number
- 2B5
- Concentration
- 0.2-1.0 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of STKL1 expressed in transfected 293T cell line by anti-STKL1 monoclonal antibody (Product # MA5-23149), clone 2B5. Lane 1: STKL1 transfected lysate (75.5 KDa). Lane 2: Non-transfected lysate.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of STKL1 over-expressed 293 cell line, cotransfected with STKL1 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with STKL1 monoclonal antibody (M03), clone 2B5 (Product # MA5-23149). GAPDH (36.1 kDa) used as specificity and loading control.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged STKL1 using anti-STKL1 monoclonal antibody (Product # MA5-23149) is approximately 0.03 ng/mL as a capture antibody.
