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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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- Product number
- MAB9548-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse Kir2.1 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human Kir2.1 in direct ELISAs. Detects human and mouse Kir2.1 in Immunohistochemistry.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Conjugate
- Unconjugated
- Antigen sequence
P63252- Isotype
- IgG
- Antibody clone number
- 2153C
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human Kir2.1 by Western Blot. Western blot shows lysates of Human heart ventricle tissue and human lung tissue. PVDF membrane was probed with 3 µg/mL of Rabbit Anti-Human/Mouse Kir2.1 Monoclonal Antibody (Catalog # MAB9548) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Kir2.1 at approximately 50-55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Kir2.1 in Human Cardiomyocytes. Kir2.1 was detected in immersion fixed human cardiomyocytes using Rabbit Anti-Human/Mouse Kir2.1 Monoclonal Antibody (Catalog # MAB9548) at 2 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Kir2.1 in Human Heart. Kir2.1 was detected in immersion fixed paraffin-embedded sections of human heart using Rabbit Anti-Human/Mouse Kir2.1 Monoclonal Antibody (Catalog # MAB9548) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to intercalated discs. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Kir2.1 in Mouse Heart. Kir2.1 was detected in perfusion fixed frozen sections of mouse heart using Rabbit Anti-Human/Mouse Kir2.1 Monoclonal Antibody (Catalog # MAB9548) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to intercalated discs. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
