MA5-45415

antibody from Invitrogen Antibodies

Targeting: KCNJ2 IRK1, Kir2.1, LQT7

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-45415

Provider

Invitrogen Antibodies

Product name

Kir2.1 (KCNJ2) Monoclonal Antibody (S112), APC

Antibody type

Monoclonal

Antigen

Other

Reactivity

Human, Mouse, Rat, Simian

Host

Mouse

Isotype

IgG

Antibody clone number

S112

Vial size

100 µg

Concentration

1 mg/mL

Storage

4° C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of Monkey COS transient cell lysate showing detection of Kir2.1 Potassium Channel protein.1 Potassium Channel. Load: 15 µg. Blocking: 1.5% BSA for 30 minutes at RT. Samples were incubated with Kir2.1 Potassium Channel monoclonal antibody (Product # MA5-45415) at 1:1,000 for 2 hours at RT, followed by Sheep Anti-Mouse IgG: HRP for 1 hour at RT.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry/Immunofluorescence analysis using human neuroblastoma cells. Fixation involved 4% PFA for 15 min. Samples were incubated with Kir2.1 monoclonal antibody (Product # MA5-45415) at 1:50 for overnight at 4°C with slow rocking, followed by AlexaFluor 488 at 1:1,000 for 1 hour at RT. Counterstain used was Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Kir2.1 antibody (D) Composite.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis using mouse backskin. Fixation involved Bouins Fixative and paraffin-embedded. Samples were incubated with Kir2.1 Potassium Channel monoclonal antibody (Product # MA5-45415) at 1:100 for 1 hour at RT, followed by FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Nuclear expression in the epidermis and hair follicles.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis using hippocampus. Fixation involved Bouins Fixative and paraffin-embedded. Samples were incubated with Kir2.1 Potassium Channel monoclonal antibody (Product # MA5-45415) at 1:1,000 for 1 hour at RT, followed by FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.