PAB29385

antibody from Abnova Corporation

Targeting: NF2 ACN, BANF, merlin, SCH

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB29385

Provider

Abnova Corporation

Product name

NF2 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant human NF2.

Antigen sequence

ERRAKQKLLEIATKPTYPPMNPIPAPLPPDIPSFN
LIGDSLSFDFKDTDMKRLSMEIEKEKVEYMEKSKH
LQEQLNELKTEIEALKLKERETALDILHNENSDRG
GSSKHNTIKKLTLQSAKSRVA


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells) Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells) with NF2 polyclonal antibody (Cat # PAB29385) at 1:500-1:1000 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-251 MG with NF2 polyclonal antibody (Cat # PAB29385) at 1-4 ug/mL concentration shows positivity in plasma membrane and cytoplasm.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human cerebral cortex with NF2 polyclonal antibody (Cat # PAB29385) shows strong cytoplasmic positivity in neuronal cells at 1:200-1:500 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)