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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [5]
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- Product number
- TA500381 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA500381, RRID:AB_2208624
- Product name
- TRPM4 mouse monoclonal antibody, clone OTI10H5 (formerly 10H5)
- Antibody type
- Monoclonal
- Description
- TRPM4 mouse monoclonal antibody, clone OTI10H5 (formerly 10H5)
- Reactivity
- Canine
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- TRPM4
- Isotype
- IgG
- Antibody clone number
- OTI10H5
- Vial size
- 100 µl
- Concentration
- 0.77 mg/ml
Submitted references Increases in reactive oxygen species enhance vascular endothelial cell migration through a mechanism dependent on the transient receptor potential melastatin 4 ion channel.
Transient receptor potential melastatin 4 inhibition prevents lipopolysaccharide-induced endothelial cell death.
Sarmiento D, Montorfano I, Cerda O, Cáceres M, Becerra A, Cabello-Verrugio C, Elorza AA, Riedel C, Tapia P, Velásquez LA, Varela D, Simon F
Microvascular research 2015 Mar;98:187-96
Microvascular research 2015 Mar;98:187-96
Transient receptor potential melastatin 4 inhibition prevents lipopolysaccharide-induced endothelial cell death.
Becerra A, Echeverría C, Varela D, Sarmiento D, Armisén R, Nuñez-Villena F, Montecinos M, Simon F
Cardiovascular research 2011 Sep 1;91(4):677-84
Cardiovascular research 2011 Sep 1;91(4):677-84
No comments: Submit comment
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-TRPM4 monoclonal antibody.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TRPM4 (RC216888, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TRPM4.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Figure from citation: Western Blot of TRPM4 protein level by using anti-TRPM4 antibody in human endothelial cells.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-TRPM4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500381)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-TRPM4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500381)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-TRPM4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500381)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-TRPM4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500381)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-TRPM4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500381)
- Validation comment
- IHC
