HPA050565

antibody from Atlas Antibodies

Targeting: DNAI2 CILD9, DIC2

Immunohistochemistry (Enhanced validation)

Antibody Data

Product number

HPA050565

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA050565, RRID:AB_2681177

Product name

Anti-DNAI2

Antibody type

Polyclonal

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

IVYVYVKKRSEFGKQCNFSDRQAELNIDIMPNPEL
AEQFVERNPVDTGIQCSISMSEHEANSERFEMETR
GVNHVEGGWPKDVNPLELEQTIRFRKKVEKDENYV
NAIMQLGSIMEHCIKQNNAI


Isotype

IgG

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Immunohistochemistry

Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Orthogonal validation

Main image

Experimental details

Immunohistochemistry analysis in human fallopian tube and prostate tissues using HPA050565 antibody. Corresponding DNAI2 RNA-seq data are presented for the same tissues.

Sample type

HUMAN

Orthogonal method

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

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Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human fallopian tube shows high expression.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human prostate shows low expression as expected.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human fallopian tube shows strong positivity in cilia in glandular cells.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human testis shows moderate membranous and cytoplasmic positivity in cells in seminiferous ducts.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human skin shows no positivity in plasma membrane in keratinocytes as expected.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human bronchus shows strong positivity in cilia in respiratory epithelial cells.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human prostate shows no positivity in glandular cells as expected.

Sample type

HUMAN