H00064446-M01

antibody from Novus Biologicals

Targeting: DNAI2 CILD9, DIC2

Provider product page for H00064446-M01

Western blot

ELISA

Immunocytochemistry

Functional assay

Antibody data

Antibody Data
Antigen structure
References [12]
Comments [0]
Validations
Western blot [2]
ELISA [1]
Immunocytochemistry [1]

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Product number: H00064446-M01 - Provider product page
Provider: Novus Biologicals
Proper citation: Novus Cat#H00064446-M01, RRID:AB_2096673
Product name: Mouse Monoclonal Dynein intermediate chain 2 Antibody
Antibody type: Monoclonal
Description: IgG purified. DNAI2 - dynein, axonemal, intermediate polypeptide 2
Reactivity: Human, Mouse, Canine
Host: Mouse
Isotype: IgG
Vial size: 0.1 mg
Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

DNAI2 protein structure - H00064446-M01 shown in red.

Submitted references DNAH2 is a novel candidate gene associated with multiple morphological abnormalities of the sperm flagella.

Li Y, Sha Y, Wang X, Ding L, Liu W, Ji Z, Mei L, Huang X, Lin S, Kong S, Lu J, Qin W, Zhang X, Zhuang J, Tang Y, Lu Z
Clinical genetics 2019 May;95(5):590-600

Vertebrate Dynein-f depends on Wdr78 for axonemal localization and is essential for ciliary beat.

Zhang Y, Chen Y, Zheng J, Wang J, Duan S, Zhang W, Yan X, Zhu X
Journal of molecular cell biology 2019 May 1;11(5):383-394

ZMYND10 stabilizes intermediate chain proteins in the cytoplasmic pre-assembly of dynein arms.

Cho KJ, Noh SH, Han SM, Choi WI, Kim HY, Yu S, Lee JS, Rim JH, Lee MG, Hildebrandt F, Gee HY
PLoS genetics 2018 Mar;14(3):e1007316

PLEKHA7 defines an apical junctional complex with cytoskeletal associations and miRNA-mediated growth implications.

Kourtidis A, Anastasiadis PZ
Cell cycle (Georgetown, Tex.) 2016;15(4):498-505

Mutations in the Motile Cilia Gene DNAAF1 Are Associated with Neural Tube Defects in Humans.

Miao C, Jiang Q, Li H, Zhang Q, Bai B, Bao Y, Zhang T
G3 (Bethesda, Md.) 2016 Oct 13;6(10):3307-3316

HEATR2 plays a conserved role in assembly of the ciliary motile apparatus.

Diggle CP, Moore DJ, Mali G, zur Lage P, Ait-Lounis A, Schmidts M, Shoemark A, Garcia Munoz A, Halachev MR, Gautier P, Yeyati PL, Bonthron DT, Carr IM, Hayward B, Markham AF, Hope JE, von Kriegsheim A, Mitchison HM, Jackson IJ, Durand B, Reith W, Sheridan E, Jarman AP, Mill P
PLoS genetics 2014 Sep;10(9):e1004577

Pih1d3 is required for cytoplasmic preassembly of axonemal dynein in mouse sperm.

Dong F, Shinohara K, Botilde Y, Nabeshima R, Asai Y, Fukumoto A, Hasegawa T, Matsuo M, Takeda H, Shiratori H, Nakamura T, Hamada H
The Journal of cell biology 2014 Jan 20;204(2):203-13

Mutations in DNAH1, which encodes an inner arm heavy chain dynein, lead to male infertility from multiple morphological abnormalities of the sperm flagella.

Ben Khelifa M, Coutton C, Zouari R, Karaouzène T, Rendu J, Bidart M, Yassine S, Pierre V, Delaroche J, Hennebicq S, Grunwald D, Escalier D, Pernet-Gallay K, Jouk PS, Thierry-Mieg N, Touré A, Arnoult C, Ray PF
American journal of human genetics 2014 Jan 2;94(1):95-104

DYX1C1 is required for axonemal dynein assembly and ciliary motility.

Tarkar A, Loges NT, Slagle CE, Francis R, Dougherty GW, Tamayo JV, Shook B, Cantino M, Schwartz D, Jahnke C, Olbrich H, Werner C, Raidt J, Pennekamp P, Abouhamed M, Hjeij R, Köhler G, Griese M, Li Y, Lemke K, Klena N, Liu X, Gabriel G, Tobita K, Jaspers M, Morgan LC, Shapiro AJ, Letteboer SJ, Mans DA, Carson JL, Leigh MW, Wolf WE, Chen S, Lucas JS, Onoufriadis A, Plagnol V, Schmidts M, Boldt K, UK10K, Roepman R, Zariwala MA, Lo CW, Mitchison HM, Knowles MR, Burdine RD, Loturco JJ, Omran H
Nature genetics 2013 Sep;45(9):995-1003

KIF5C, a kinesin motor involved in apical trafficking of MDCK cells.

Astanina K, Jacob R
Cellular and molecular life sciences : CMLS 2010 Apr;67(8):1331-42

DNAI2 mutations cause primary ciliary dyskinesia with defects in the outer dynein arm.

Loges NT, Olbrich H, Fenske L, Mussaffi H, Horvath J, Fliegauf M, Kuhl H, Baktai G, Peterffy E, Chodhari R, Chung EM, Rutman A, O'Callaghan C, Blau H, Tiszlavicz L, Voelkel K, Witt M, Zietkiewicz E, Neesen J, Reinhardt R, Mitchison HM, Omran H
American journal of human genetics 2008 Nov;83(5):547-58

Ktu/PF13 is required for cytoplasmic pre-assembly of axonemal dyneins.

Omran H, Kobayashi D, Olbrich H, Tsukahara T, Loges NT, Hagiwara H, Zhang Q, Leblond G, O'Toole E, Hara C, Mizuno H, Kawano H, Fliegauf M, Yagi T, Koshida S, Miyawaki A, Zentgraf H, Seithe H, Reinhardt R, Watanabe Y, Kamiya R, Mitchell DR, Takeda H
Nature 2008 Dec 4;456(7222):611-6

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Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: Dynein intermediate chain 2 Antibody (1C8) [H00064446-M01] - Analysis of DNAI2 expression in transfected 293T cell line by DNAI2 monoclonal antibody (M01), clone 1C8.Lane 1: DNAI2 transfected lysate(67 KDa).Lane 2: Non-transfected lysate.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: Dynein intermediate chain 2 Antibody (1C8) [H00064446-M01] - DNAI2 monoclonal antibody (M01), clone 1C8 Analysis of DNAI2 expression in A-431.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Sandwich ELISA: Dynein intermediate chain 2 Antibody (1C8) [H00064446-M01] - Detection limit for recombinant GST tagged DNAI2 is approximately 0.03ng/ml as a capture antibody.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Immunocytochemistry/Immunofluorescence: Dynein intermediate chain 2 Antibody (1C8) [H00064446-M01] - Analysis of monoclonal antibody to DNAI2 on A-431 cell. Antibody concentration 10 ug/ml.