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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Flow cytometry [1]
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- Product number
- AF3138 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Granulysin Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human Granulysin in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human NKG-2D is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
P22749- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Detection of Granulysin in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells were treated for 24 hr with 50 ng/ml PMA and 200 ng/ml Ca2+ ionomycin then stained with Goat Anti-Human Granulysin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3138, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).
