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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
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- Product number
- H00010487-M01 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00010487-M01, RRID:AB_464325
- Product name
- CAP1 monoclonal antibody (M01), clone 4A2
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full length recombinant CAP1.
- Antigen sequence
MADMQNLVERLERAVGRLEAVSHTSDMHRGYADSP
SKAGAAPYVQAFDSLLAGPVAEYLKISKEIGGDVQ
KHAEMVHTGLKLERALLVTASQCQQPAENKLSDLL
APISEQIKEVITFREKNRGSKLFNHLSAVSESIQA
LGWVAMAPKPGPYVKEMNDAAMFYTNRVLKEYKDV
DKKHVDWVKAYLSIWTELQAYIKEFHTTGLAWSKT
GPVAKELSGLPSGPSAGSGPPPPPPGPPPPPVSTS
SGSDESASRSALFAQINQGESITHALKHVSDDMKT
HKNPALKAQSGPVRSGPKPFSAPKPQTSPSPKRAT
KKEPAVLELEGKKWRVENQENVSNLVIEDTELKQV
AYIYKCVNTTLQIKGKINSITVDNCKKLGLVFDDV
VGIVEIINSKDVKVQVMGKVPTISINKTDGCHAYL
SKNSLDCEIVSAKSSEMNVLIPTEGGDFNEFPVPE
QFKTLWNGQKLVTTVTEIAG- Isotype
- IgG
- Antibody clone number
- 4A2
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Submitted references Expression and function of neuronal growth-associated proteins (nGAPs) in PC12 cells.
Adenylyl cyclase-associated protein-1/CAP1 as a biological target substrate of gelatinase B/MMP-9.
Lu J, Nozumi M, Takeuchi K, Abe H, Igarashi M
Neuroscience research 2011 May;70(1):85-90
Neuroscience research 2011 May;70(1):85-90
Adenylyl cyclase-associated protein-1/CAP1 as a biological target substrate of gelatinase B/MMP-9.
Cauwe B, Martens E, Van den Steen PE, Proost P, Van Aelst I, Blockmans D, Opdenakker G
Experimental cell research 2008 Sep 10;314(15):2739-49
Experimental cell research 2008 Sep 10;314(15):2739-49
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- CAP1 monoclonal antibody (M01), clone 4A2 Western Blot analysis of CAP1 expression in HeLa ( Cat # L013V1 ).
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged CAP1 is approximately 0.3ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
