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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [4]
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Validation data
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- Product number
- 13731-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Carboxypeptidase A5 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: RLERSTNSF SYSSYHTLEE IYSWIDNFVM EHSDIVSKIQ IGNSFENQSI LVLKFSTGGS RHPAIWIDTG IHSREWITHA TGIWTANKIV SDYGKDRVLT DILNAMDIFI ELVTNPDGFA FTHSMNRLWR KNKSIRPGIF CIGVDLNRNW KSGFGGNGSN SNPCSETYHG PSPQSEPEVA AIVNFITAHG NFKALISIHS YSQMLMYPYG RLLEPVSNQR ELYDLAKDAV EALYKVHGIE YIFGSISTTL YVASGITVDW AYDSGIKYAF SFELRDTGQY GFLLPATQII PTAQETWMAL RTIMEHTLNH PY (126-436 aa encoded by BC039362)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.13 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- PC-3 cells were subjected to SDS PAGE followed by western blot with 13731-1-AP (Carboxypeptidase A5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of PC-3 cells using 13731-1-AP (Carboxypeptidase A5 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of PC-3 cells using 13731-1-AP (Carboxypeptidase A5 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded mouse testis tissue slide using 13731-1-AP (Carboxypeptidase A5 antibody) at dilution of 1:100 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded mouse testis tissue slide using 13731-1-AP (Carboxypeptidase A5 antibody) at dilution of 1:100 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human testis tissue slide using 13731-1-AP (Carboxypeptidase A5 antibody) at dilution of 1:100 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human testis tissue slide using 13731-1-AP (Carboxypeptidase A5 antibody) at dilution of 1:100 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
