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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [4]
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Validation data
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- Product number
- 27979-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NOSIP Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: MTRHGKNCT AGAVYTYHEK KKDTAASGYG TQNIRLSRDA VKDFDCCCLS LQPCHDPVVT PDGYLYEREA ILEYILHQKK EIARQMKAYE KQRGTRREEQ KELQRAASQD HVRGFLEKES AIVSRPLNPF TAKALSGTSP DDVQPGPSVG PPSKDKDKVL PSFWIPSLTP EAKATKLEKP SRTVTCPMSG KPLRMSDLTP VHFTPLDSSV DRVGLITRSE RYVCAVTRDS LSNATPCAVL RPSGAVVTLE CVEKLIRKDM VDPVTGDKLT DRDIIVLQRG GTGFAGSGVK LQAEKSRPVM QA (1-301 aa encoded by BC010077)
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.27 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Various lysates were subjected to SDS PAGE followed by western blot with 27979-1-AP (NOSIP antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- A431 cells were subjected to SDS PAGE followed by western blot with 27979-1-AP (NOSIP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ( 4% PFA) fixed HeLa cells using 27979-1-AP (NOSIP antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human lung tissue slide using 27979-1-AP (NOSIP antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human lung tissue slide using 27979-1-AP (NOSIP antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human colon tissue slide using 27979-1-AP (NOSIP antibody) at dilution of 1:200 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human colon tissue slide using 27979-1-AP (NOSIP antibody) at dilution of 1:200 (under 40x lens).
