TA503259

antibody from OriGene

Targeting: DHFR2 DHFRL1, DHFRP4, FLJ16119

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA503259

Provider

OriGene

Proper citation

OriGene Cat#TA503259, RRID:AB_11124830

Product name

DHFRL1 mouse monoclonal antibody, clone OTI1E2 (formerly 1E2)

Antibody type

Monoclonal

Description

DHFRL1 mouse monoclonal antibody, clone OTI1E2 (formerly 1E2)

Reactivity

Canine

Host

Mouse

Conjugate

Unconjugated

Epitope

DHFR2

Isotype

IgG

Antibody clone number

OTI1E2

Vial size

100 µl

Concentration

0.73 mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-DHFRL1 monoclonal antibody.

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DHFRL1 (RC208452, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DHFRL1.

Validation comment

WB

Immunocytochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Anti-DHFRL1 mouse monoclonal antibody (TA503259) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY DHFRL1(RC208452).

Validation comment

IF