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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- AP50301PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP50301PU-N, RRID:AB_11148238
- Product name
- anti ATP synthase subunit d (Center)
- Antibody type
- Polyclonal
- Antigen
- KLH conjugated synthetic peptide between 75-105 amino acids from the Central region of human ATP5H
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 0.4 ml
- Concentration
- lot specific
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- ATP5H Antibody (Center) (Cat. #AP50301PU-N) western blot analysis in HepG2 cell line lysates (35µg/lane).This demonstrates the ATP5H antibody detected the ATP5H protein (arrow).
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- ATP5H antibody (Center) (Cat. #AP50301PU-N) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ATP5H antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- ATP5H Antibody (Center) (Cat. #AP50301PU-N) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
