M09565

antibody from Boster Biological Technology

Targeting: ATP5PD ATP5H, ATP5JD, ATPQ

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

M09565

Provider

Boster Biological Technology

Product name

Anti-ATP5H in Antibody Picoband™ (monoclonal, 6B12)

Antibody type

Monoclonal

Description

Mouse IgG monoclonal antibody for ATP5H detection. Tested with WB, IHC-P, ICC/IF in Human;Rat;Mouse;Monkey.

Reactivity

Human, Mouse, Rat, Simian

Host

Mouse

Isotype

IgG

Antibody clone number

6B12

Vial size

100μg/vial

Storage

At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

Handling

Add 0.2ml of distilled water will yield a concentration of 500μg/ml.

Western blot

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

Western blot analysis of ATP5H using anti-ATP5H antibody (M09565). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela tissue lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human U-87MG whole cell lysates, Lane 4: human A549 whole cell lysates, Lane 5: monkey COS-7 whole cell lysates, Lane 6: human SW620 whole cell lysates. Lane 7: rat PC-12 whole cell lysates. Lane 8: mouse Ana-1 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti- ATP5H antigen affinity purified polyclonal antibody (Catalog # M09565) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for ATP5H at approximately 22KD. The expected band size for ATP5H is at 18KD.

Additional image