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LearnNBP2-24570
antibody from Novus Biologicals
Targeting: EIF3A
EIF3, eIF3-p170, eIF3-theta, EIF3S10, KIAA0139, TIF32
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- NBP2-24570 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal EIF3A Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: EIF3A Antibody [NBP2-24570] - analysis of eIF3A in Daudi lysate in the 1) absence and 2) presence of immunizing peptide and 3) NIH 3T3 lysate using eIF3A antibody at 0.5 ug/ml, 0.5 ug/ml and 0.25 ug/ml respectively.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: EIF3A Antibody [NBP2-24570] - Simple Western lane view shows a specific band for EIF3A in 0.5 mg/ml of NIH-3T3 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: EIF3A Antibody [NBP2-24570] - Formalin-fixed, paraffin-embedded human brain tissue stained with eIF3A antibody at 10 ug/ml. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
