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LearnPA5-23285
antibody from Invitrogen Antibodies
Targeting: EIF3A
EIF3, eIF3-p170, eIF3-theta, EIF3S10, KIAA0139, TIF32
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [1]
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- Product number
- PA5-23285 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- eIF3a Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of eIF3a in 0.5 mg/mL NIH-3T3 lysate. Samples were incubated in eIF3a polyclonal antibody (Product # PA5-23285). This experiment was performed under reducing conditions using the 12-230 kDa separation system.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of eIF3a in Daudi lysate in the 1) absence and 2) presence of immunizing peptide and 3) NIH 3T3 lysate. Samples were incubated in eIF3a polyclonal antibody (Product # PA5-23285) using a dilution of 0.5 µg/mL, 0.5 µg/mL and 0.25 µg/mL respectively.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of eIF3a in 0.5 mg/mL NIH-3T3 lysate. Samples were incubated in eIF3a polyclonal antibody (Product # PA5-23285). This experiment was performed under reducing conditions using the 12-230 kDa separation system.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of eIF3a in formalin-fixed, paraffin-embedded human brain tissue. Samples were incubated in eIF3a polyclonal antibody (Product # PA5-23285) using a dilution of 10 µg/mL. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
