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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- GTX50000 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX50000, RRID:AB_11169199
- Product name
- SUMO2 antibody [AT10F1]
- Antibody type
- Monoclonal
- Reactivity
- Human
- Host
- Mouse
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Cell lysates of HeLa (35ug) and recombinant proteins (10ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human SUMO2/3 (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an EC
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Cell lysates of HeLa (35ug) and recombinant proteins (10ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human SUMO2/3 (1:500, GTX50000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunofluorescence of human HeLa cells stained with Hoechst 3342 (Blue) for nucleus staining and monoclonal anti-human SUMO2/3 antibody (1:500) with Texas Red (Red).
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Paraffin embedded sections of colorectal cancer tissue were incubated with anti-human SUMO2/3 antibody (1:50, GTX50000) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB).
