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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunocytochemistry [1]
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- Product number
- BML-PW9465-0100 - Provider product page
- Provider
- Enzo Life Sciences
- Product name
- SUMO-2/3 (human) (NT) polyclonal antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Partially purified by salt precipitation.
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 100 μl
- Storage
- -20°C
Submitted references ATPase-dependent control of the Mms21 SUMO ligase during DNA repair.
Bermúdez-López M, Pociño-Merino I, Sánchez H, Bueno A, Guasch C, Almedawar S, Bru-Virgili S, Garí E, Wyman C, Reverter D, Colomina N, Torres-Rosell J
PLoS biology 2015 Mar;13(3):e1002089
PLoS biology 2015 Mar;13(3):e1002089
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Supportive validation
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- Enzo Life Sciences (provider)
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- Experimental details
- Western Blot Analysis: SUMOylation assay utilising His6-tagged SUMO-2 (BML-UW9205), SUMO E1 (BML-UW9330), SUMO E2 (BML-UW9320) and RANGAP1 as substrate in presence (lane 2) and absence (lanes 1) of ATP after SDS-PAGE and blotting to PVDF with subsequent probing with BML-PW9465.
