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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [4]
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Validation data
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- Product number
- 19370-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NUP85 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: FLVTRLLYS NPTVKPIDLH YYAQSSLDLF LGGESSPEPL DNILLAAFEF DIHQVIKECS IALSNWWFVA HLTDLLDHCK LLQSHNLYFG SNMREFLLLE YASGLFAHPS LWQLGVDYFD YCPELGRVSL ELHIERIPLN TEQKALKVLR ICEQRQMTEQ VRSICKILAM KAVRNNRLGS ALSWSIRAKD AAFATLVSDR FLRDYCERGC FSDLDLIDNL GPAMMLSDRL TFLGKYREFH RMYGEKRFAD AASLLLSLMT SRIAPRSFWM TLLTDALPLL EQKQVIFSAE QTYELMRCLE DLTSRRPVHG ESDTEQLQDD DIETTKVEML RLSLARNLAR AIIREGSLEG S (307-656 aa encoded by BC000697)
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.16 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Human brain tissue were subjected to SDS PAGE followed by western blot with 19370-1-AP (NUP85 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse lung tissue were subjected to SDS PAGE followed by western blot with 19370-1-AP (NUP85 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 19370-1-AP (NUP85 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IF result (Nucleus stain) of anti-NUP85 (19370-1-AP; 1:50) hTERT-RPE1 cell (MeOH fixed) by Dr. Moshe Kim.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human liver using 19370-1-AP (NUP85 antibody) at dilution of 1:50 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human liver using 19370-1-AP (NUP85 antibody) at dilution of 1:50 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human stomach using 19370-1-AP (NUP85 antibody) at dilution of 1:50 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human stomach using 19370-1-AP (NUP85 antibody) at dilution of 1:50 (under 40x lens).
