Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- AF5976 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Nucleoporin NUP85 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human NUP85 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse NUP85 is observed.
- Reactivity
- Human
- Host
- Sheep
- Conjugate
- Unconjugated
- Antigen sequence
Q9BW27- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
No comments: Submit comment
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human Nucleoporin NUP85 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Nucleoporin NUP85 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5976) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Nucleoporin NUP85 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Nucleoporin NUP85 in Human MSCs. Nucleoporin NUP85 was detected in immersion fixed human multipotent stromal cells (MSCs) using Sheep Anti-Human Nucleoporin NUP85 Antigen Affinity-purified Poly-clonal Antibody (Catalog # AF5976) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the Northern-Lights™ 493-conjugated Anti-Sheep IgG Secondary Antibody (green; Catalog # NL012) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
