847402

antibody from BioLegend

Targeting: ATG5 APG5, APG5L, ASP, hAPG5

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

847402

Provider

BioLegend

Proper citation

BioLegend Cat#847402, RRID:AB_2629668

Product name

Purified anti-ATG5

Antibody type

Monoclonal

Antigen

ATG5 peptide conjugated to KLH.

Reactivity

Human, Mouse

Host

Mouse

Conjugate

Unconjugated

Antibody clone number

177.19

Vial size

100 µg

Concentration

0.5 μ/ml

Storage

2°C-8°C

Handling

Ambient RT

Western blot

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

Western blot of anti-ATG5 antibody (clone 177.19) demonstrating detection of endogenous ATG5 in brain tissue and cell lysates. Lane 1: 30 µg of Hela lysate, Lane 2: 30 µg of TSA-treated Hela lysate, Lane 3: 30 µg of Nocodazole-treated Hela lysate, Lane 4: 30 µg of human brain lysate, Lane 5: 30 µg of mouse brain lysate. The Blot was incubated with the primary antibody overnight at 4°C at 5 µg/ml, followed by incubation with HRP labeled goat anti-mouse secondary antibody.

Conjugate

Unconjugated