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Learn25625-1-AP
antibody from Invitrogen Antibodies
Targeting: CHCHD3
FLJ20420, Mic19, MICOS19, MINOS3, PPP1R22
Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Immunohistochemistry [4]
- Other assay [1]
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- Product number
- 25625-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CHCHD3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: MGGTTSTRR VTFEADENEN ITVVKGIRLS ENVIDRMKES SPSGSKSQRY SGAYGASVSD EELKRRVAEE LALEQAKKES EDQKRLKQAK ELDRERAAAN EQLTRAILRE RICSEEERAK AKHLARQLEE KDRVLKKQDA FYKEQLARLE ERSSEFYRVT TEQYQKAAEE VEAKFKRYES HPVCADLQAK ILQCYRENTH QTLKCSALAT QYMHCVNHAK QSMLEKGG (1-227 aa encoded by BC014839)
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.34 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK-293 cells were subjected to SDS PAGE followed by western blot with 25625-1-AP (CHCHD3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- A431 cells were subjected to SDS PAGE followed by western blot with 25625-1-AP (CHCHD3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 25625-1-AP ( CHCHD3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse liver tissue were subjected to SDS PAGE followed by western blot with 25625-1-AP (CHCHD3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HepG2 cells using 25625-1-AP (CHCHD3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human heart tissue slide using 25625-1-AP (CHCHD3 Antibody) at dilution of 1:50 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human heart tissue slide using 25625-1-AP (CHCHD3 Antibody) at dilution of 1:50 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human liver tissue slide using 25625-1-AP ( CHCHD3 Antibody) at dilution of 1:50 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human liver tissue slide using 25625-1-AP ( CHCHD3 Antibody) at dilution of 1:50 (under 40x lens).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-CHCHD3 (IP:25625-1-AP, 4ug; Detection:25625-1-AP 1:1000) with HeLa cells lysate 2600ug.
