Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [5]
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Validation data
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- Product number
- NBP1-82456 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-82456, RRID:AB_11040668
- Product name
- Rabbit Polyclonal SEC23IP Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human SEC23IP antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian cells.
Stadler C, Rexhepaj E, Singan VR, Murphy RF, Pepperkok R, Uhlén M, Simpson JC, Lundberg E
Nature methods 2013 Apr;10(4):315-23
Nature methods 2013 Apr;10(4):315-23
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SEC23IP Antibody [NBP1-82456] - Analysis in A-549 cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SEC23IP Antibody [NBP1-82456] - Analysis using Anti-SEC23IP antibody NBP1-82456 (A) shows similar pattern to independent antibody NBP2-58361 (B).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SEC23IP Antibody [NBP1-82456] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SEC23IP Antibody [NBP1-82456] - Staining of human stomach shows strong cytoplasmic positivity in glandular cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SEC23IP Antibody [NBP1-82456] - Staining of human skeletal muscle shows no positivity in myocytes as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SEC23IP Antibody [NBP1-82456] - Staining of human stomach shows strong granular cytoplasmic positivity in glandular cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SEC23IP Antibody [NBP1-82456] - Staining of human testis shows moderate granular cytoplasmic positivity in cells in seminiferous ducts.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SEC23IP Antibody [NBP1-82456] - Staining of human tonsil shows moderate granular cytoplasmic positivity in germinal center cells.