Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- MAB6408 - Provider product page
- Provider
- R&D Systems
- Product name
- Human PRAS40 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human PRAS40 in Western blots.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
Q96B36- Isotype
- IgG
- Antibody clone number
- 660928
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Soluble bone-derived osteopontin promotes migration and stem-like behavior of breast cancer cells.
Pio GM, Xia Y, Piaseczny MM, Chu JE, Allan AL
PloS one 2017;12(5):e0177640
PloS one 2017;12(5):e0177640
No comments: Submit comment
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human PRAS40 by Western Blot. Western blot shows lysates of 293T human embryonic kidney cell line, A549 human lung carcinoma cell line, MDA-MB-468 human breast cancer cell line, and T47D human breast cancer cell line. PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human PRAS40 Monoclonal Antibody (Catalog # MAB6408) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for PRAS40 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human PRAS40 by Simple WesternTM. Simple Western lane view shows lysates of 293T human embryonic kidney cell line, loaded at 0.5 mg/mL. A specific band was detected for PRAS40 at approximately 65 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human PRAS40 Monoclonal Antibody (Catalog # MAB6408). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- PRAS40 in HeLa Human Cell Line. PRAS40 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human PRAS40 Monoclonal Antibody (Catalog # MAB6408) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
