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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- BS4760 - Provider product page
- Provider
- Bioworld Technology, Inc
- Proper citation
- Bioworld Technology Cat#BS4760, RRID:AB_1663165
- Product name
- Histone H2A.X (phospho S139) polyclonal antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic phosphopeptide derived from human Histone H2A.X around the phosphorylation site of Serine 139.
- Description
- H2A.X is a member of the histone H2A family, 1 of 5 families of histone proteins involved in nucleosomal organization of chromatin. H2A.X is synthesised in G1 as well as S phase and is known to be important for recombination between immunoglobulin switch regions. A very early step in the response of mammalian cells to DNA double-strand breaks is the phosphorylation of histone H2A.X at serine 139 at the sites of DNA damage. There is a predicted acetylation at residue 1 and ubiquitination at reside 119. Phosphorylated H2A.X promotes DNA repair and maintains genomic stability.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100ul
- Concentration
- 1 mg/ml
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
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Supportive validation
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- Bioworld Technology, Inc (provider)
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- Experimental details
- Western blot (WB) analysis of p-Histone H2A.X (S139) pAb at 1:500 dilutionLane1:Hela cell lysate treated with colchicine(0.2¦Ìg/ML,24h)Lane2:sp2/0 cell lysate treated with colchicine(0.2¦Ìg/ML,24h)Lane3:PC12 cell lysate treated with colchicine(0.2¦Ìg/ML,24h)
