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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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Validation data
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- Product number
- LS-C287019 - Provider product page
- Provider
- LSBio
- Product name
- H2AFX / H2AX Antibody (phospho-Ser139, Biotin) LS-C287019
- Antibody type
- Polyclonal
- Description
- Immunoaffinity purified
- Reactivity
- Human, Mouse, Bovine, Canine, Guinea Pig, Porcine, Rabbit
- Host
- Rabbit
- Conjugate
- Biotin
- Isotype
- IgG
- Storage
- Store at 4°C. Stable for 6 months.
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Supportive validation
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- LSBio (provider)
- Main image
- Experimental details
- Detection of Human gamma-H2AX by Western Blot. Samples: Whole cell lysate from etoposide treated HeLa (50, 15, and 5 ug). Antibody: Affinity purified, biotin-conjugated, rabbit anti-gamma-H2AX antibody used at 1.0 ug/ml. Detection: Streptavidin-HRP and chemiluminescence with an exposure time of 30 seconds. This image was taken for the unconjugated form of this product. Other forms have not been tested.
