LS-C290487

antibody from LSBio

Targeting: H2AX H2AFX

Western blot (Enhanced validation)

Immunohistochemistry (Enhanced validation)

Antibody Data

Product number

LS-C290487

Provider

LSBio

Product name

H2AFX / H2AX Antibody (C-Terminus) LS-C290487

Antibody type

Polyclonal

Description

Affinity purified

Reactivity

Human, Mouse

Host

Goat

Isotype

IgG

Storage

Store at 2-8°C for up to one year.

Western blot

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Detection of Human H2AX by Western Blot. Samples: Whole cell lysate from 293T (15 and 50 ug) cells. Antibodies: Affinity purified goat anti-H2AX antibody used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Detection of human H2AX by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, and K-562cells prepared using RIPA lysis buffer. Antibody: Affinity purified rabbit anti-H2AX antibody used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Immunohistochemistry

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Detection of Mouse H2AX by Immunohistochemistry. Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-H2AX used at a dilution of 1:1000 (1 ug/ml). Detection: DAB.

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Detection of Human H2AX by Immunohistochemistry. Sample: FFPE section of human lung cancer. Antibody: Affinity purified rabbit anti-H2AX used at a dilution of 1:5000 (0.2 ug/ml). Detection: DAB.

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Detection of mouse H2AX by immunohistochemistry. Sample: FFPE section of mouse CT26 colon carcinoma. Antibody: Affinity purified goat anti- H2AX used at a dilution of 1:1,000 (1µg/ml). Detection: DAB

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Detection of human H2AX by immunohistochemistry. Sample: FFPE section of human ovarian cancer. Antibody: Affinity purified goat anti- H2AX used at a dilution of 1:5,000 (0.2µg/ml). Detection: DAB