TA321434

antibody from OriGene

Targeting: H2AX H2AFX

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA321434

Provider

OriGene

Product name

Anti-H2AFX (Phospho-Ser139) Rabbit Polyclonal Antibody

Antibody type

Polyclonal

Description

Anti-H2AFX (Phospho-Ser139) Rabbit Polyclonal Antibody

Host

Rabbit

Conjugate

Unconjugated

Epitope

H2AFX

Isotype

IgG

Antibody clone number

NULL

Vial size

100 µl

Concentration

1mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Predicted band size: 15 kDa . Positive control: HT29 cells untreated or treated with UV lysate. Recommended dilution: 1/ 500-1000. (Gel: 12%SDS-PAGE Lane1: HT29 cells untreated with UV lysate Lane2: HT29 cells treated with UV lysate Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)

Validation comment

WB

Immunocytochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Predicted cell location: Nucleus. Positive control: Hela cells. Recommended dilution: 1/ 100-200.The image is immunofluorescence of methanol-fixed Hela cells using H2AFX (Phospho-Ser139) antibody at dilution 1/100. (Original magnification:?—200)

Validation comment

IF