613412

antibody from BioLegend

Targeting: H2AX H2AFX

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

613412

Provider

BioLegend

Proper citation

BioLegend Cat#613412, RRID:AB_2616871

Product name

PE anti-H2A.X-Phosphorylated (Ser139)

Antibody type

Monoclonal

Antigen

Modified peptide

Reactivity

Human

Host

Mouse

Conjugate

Yellow dye

Antibody clone number

2F3

Vial size

100 tests

Storage

2°C-8°C

Handling

Ambient RT

Flow cytometry

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

Nocodozole treated HeLa cells (24 hours) were fixed and permeabilized with cold 70% ethanol, then intracellularly stained with anti-H2A.X Phospho (Ser139) (clone 2F3) PE (filled histogram) or mouse IgG1, κ PE (open histogram).

Conjugate

Yellow dye