R20231

antibody from NSJ Bioreagents

Targeting: H2AX H2AFX

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

R20231

Provider

NSJ Bioreagents

Product name

Recombinant phospho-Histone H2A/H4 Antibody (Ser1)

Antibody type

Recombinant

Description

Purified

Host

Rabbit

Conjugate

Unconjugated

Antibody clone number

RM216

Vial size

25 µg, 100 µg

Concentration

1 mg/ml in PBS with 50% Glycerol, 1% BSA and 0.09% sodium azide

Storage

Store the recombinant phospho-Histone H2A/H4 antibody at -20°C.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot test of acid extracts of HeLa cells treated (1) or non-treated (2) with Nocodazole, using recombinant phospho-Histone H2A/H4 antibody at 0.5 ug/ml, showed both Histone H2A and H4 phosphorylated at Serine 1 in HeLa cells.

Immunocytochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

ICC/IF staining of HeLa cells using recombinant phospho-Histone H2A/H4 antibody (red). Actin filaments have been labeled with fluorescein phalloidin (green).

Other assay

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

This recombinant phospho-Histone H2A/H4 antibody specifically reacts to both Histone H2A and H4 phosphorylated at Serine 1 (H2AS1p and H4S1p).