A700-053

antibody from Invitrogen Antibodies

Targeting: H2AX H2AFX

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

A700-053

Provider

Invitrogen Antibodies

Product name

gamma-H2AX Recombinant Rabbit Monoclonal Antibody (BLR053F)

Antibody type

Monoclonal

Antigen

Other

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Antibody clone number

BLR053F

Vial size

100 µL

Concentration

100 µg/mL

Storage

4° C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human gamma-H2AX by western blot. Samples: Whole cell lysate (50 µg) from Jurkat cells treated with 100 µM etoposide (+) or mock treated (-). Antibody: Rabbit anti-gamma-H2AX recombinant monoclonal antibody [BLR053F] (Product # A700-053 lot 2) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-101P). Chemiluminescence with an exposure time of 3 seconds.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human gamma-H2AX by immunocytochemistry. Sample: FFPE section of human HeLa cells treated with etoposide. Antibody: Rabbit anti-gamma-H2AX recombinant monoclonal antibody [BLR053F] (Product # A700-053 lot 2) used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate:DAB.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human gamma-H2AX by immunohistochemistry. AntibodySample: FFPE section of human basal cell carcinoma.Antibody: Rabbit anti-gamma-H2AX recombinant monoclonal antibody (BLR053F) (A700-053 lot 1) used at 1:250.Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate: DAB.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human gamma-H2AX by immunohistochemistry. Sample: FFPE section of human basal cell carcinoma. Antibody: Rabbit anti-gamma-H2AX recombinant monoclonal antibody [BLR053F] (Product # A700-053 lot 2) used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate:DAB.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of mouse gamma-H2AX by immunohistochemistry. Sample: FFPE section of mouse CT26 tumor. Antibody: Rabbit anti-gamma-H2AX recombinant monoclonal antibody [BLR053F] (Product # A700-053 lot 2) used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate:DAB.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human gamma-H2AX by immunohistochemistry.Sample: FFPE section of human breast carcinoma.Antibody: Rabbit anti-gamma-H2AX recombinant monoclonal antibody [BLR053F] (Product # A700-053 lot 1) used at 1:100.Secondary: DyLight® 594-conjugated goat anti-rabbit IgG (A120-101D4). Counterstain: DAPI.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human gamma H2AX (shaded) in etoposide treated HEK293T cells by flow cytometry. Antibody: Rabbit anti-gamma H2AX recombinant monoclonal [BLR053F] (Product #A700-053) or isotype control (unshaded). Secondary: DyLight® 488-conjugated goat anti-rabbit IgG (Product # A120-101D2).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human Gamma-H2AX by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells treated with 100 µM etoposide or mock treated (-). Antibodies: Rabbit anti-Gamma-H2AX recombinant monoclonal antibody [BLR053F] (Product # A700-053 lot 2) used for IP at 20 µL/mg lysate. H2AX was also immunoprecipitated by goat anti-H2AX antibody (Product # A303-837A). For blotting immunoprecipitated Gamma-H2AX (Product # A700-053) was used at 1:1000. Chemiluminescence with an exposure time of 3 seconds. Lower Panel: goat anti-H2AX antibody (Product # A303-837A).