A700-053-T
antibody from Invitrogen Antibodies
Targeting: H2AX
H2AFX
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [4]
- Flow cytometry [1]
- Other assay [1]
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Validation data
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- Product number
- A700-053-T - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- gamma-H2AX Recombinant Rabbit Monoclonal Antibody (BLR053F)
- Antibody type
- Monoclonal
- Antigen
- Other
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- BLR053F
- Vial size
- 10 µL
- Concentration
- 100 µg/mL
- Storage
- 4° C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human gamma-H2AX by western blot. Samples: Whole cell lysate (50 µg) from Jurkat cells treated with 100 µM etoposide (+) or mock treated (-). Antibody: Rabbit anti-gamma-H2AX recombinant monoclonal antibody [BLR053F] (Product # A700-053 lot 2) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-101P). Chemiluminescence with an exposure time of 3 seconds.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human gamma-H2AX by immunocytochemistry. Sample: FFPE section of human HeLa cells treated with etoposide. Antibody: Rabbit anti-gamma-H2AX recombinant monoclonal antibody [BLR053F] (Product # A700-053 lot 2) used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate:DAB.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human gamma-H2AX by immunohistochemistry. AntibodySample: FFPE section of human basal cell carcinoma.Antibody: Rabbit anti-gamma-H2AX recombinant monoclonal antibody (BLR053F) (A700-053 lot 1) used at 1:250.Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate: DAB.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human gamma-H2AX by immunohistochemistry. Sample: FFPE section of human basal cell carcinoma. Antibody: Rabbit anti-gamma-H2AX recombinant monoclonal antibody [BLR053F] (Product # A700-053 lot 2) used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate:DAB.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of mouse gamma-H2AX by immunohistochemistry. Sample: FFPE section of mouse CT26 tumor. Antibody: Rabbit anti-gamma-H2AX recombinant monoclonal antibody [BLR053F] (Product # A700-053 lot 2) used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate:DAB.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human gamma-H2AX by immunohistochemistry.Sample: FFPE section of human breast carcinoma.Antibody: Rabbit anti-gamma-H2AX recombinant monoclonal antibody [BLR053F] (Product # A700-053 lot 1) used at 1:100.Secondary: DyLight® 594-conjugated goat anti-rabbit IgG (A120-101D4). Counterstain: DAPI.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human gamma H2AX (shaded) in etoposide treated HEK293T cells by flow cytometry. Antibody: Rabbit anti-gamma H2AX recombinant monoclonal [BLR053F] (Product #A700-053) or isotype control (unshaded). Secondary: DyLight® 488-conjugated goat anti-rabbit IgG (Product # A120-101D2).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human Gamma-H2AX by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells treated with 100 µM etoposide or mock treated (-). Antibodies: Rabbit anti-Gamma-H2AX recombinant monoclonal antibody [BLR053F] (Product # A700-053 lot 2) used for IP at 20 µL/mg lysate. H2AX was also immunoprecipitated by goat anti-H2AX antibody (Product # A303-837A). For blotting immunoprecipitated Gamma-H2AX (Product # A700-053) was used at 1:1000. Chemiluminescence with an exposure time of 3 seconds. Lower Panel: goat anti-H2AX antibody (Product # A303-837A).