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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [90]
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- Validations
- Western blot [5]
- Immunohistochemistry [4]
- Flow cytometry [1]
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- Product number
- NB100-384 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-384, RRID:AB_10002815
- Product name
- Rabbit Polyclonal gamma H2AX Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. The epitope maps to a region surrounding phosphorylated serine 139 of human histone H2AX.
- Reactivity
- Human, Mouse, Rat, Canine
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references Collective cancer invasion forms an integrin-dependent radioresistant niche.
Association of glomerular DNA damage and DNA methylation with one-year eGFR decline in IgA nephropathy.
Randomized, double-blind trial of F14512, a polyamine-vectorized anticancer drug, compared with etoposide phosphate, in dogs with naturally occurring lymphoma.
Dietary riboflavin deficiency promotes N-nitrosomethylbenzylamine-induced esophageal tumorigenesis in rats by inducing chronic inflammation.
CTDP1 regulates breast cancer survival and DNA repair through BRCT-specific interactions with FANCI.
Methods to Quantify the NF-κB Pathway During Senescence.
Mouse Models of Accelerated Cellular Senescence.
SET Domain-Containing Protein 4 Epigenetically Controls Breast Cancer Stem Cell Quiescence.
Anti-Tumor Effects of Wee1 Kinase Inhibitor with Radiotherapy in Human Cervical Cancer.
Functional Impacts of the BRCA1-mTORC2 Interaction in Breast Cancer.
BRCA1 Haploinsufficiency Is Masked by RNF168-Mediated Chromatin Ubiquitylation.
DNA Replication Vulnerabilities Render Ovarian Cancer Cells Sensitive to Poly(ADP-Ribose) Glycohydrolase Inhibitors.
p53-TP53-Induced Glycolysis Regulator Mediated Glycolytic Suppression Attenuates DNA Damage and Genomic Instability in Fanconi Anemia Hematopoietic Stem Cells.
PARP inhibitor re‑sensitizes Adriamycin resistant leukemia cells through DNA damage and apoptosis.
Reprogramming of Meiotic Chromatin Architecture during Spermatogenesis.
A truncating mutation in the autophagy gene UVRAG drives inflammation and tumorigenesis in mice.
Systematic bromodomain protein screens identify homologous recombination and R-loop suppression pathways involved in genome integrity.
The p38α Stress Kinase Suppresses Aneuploidy Tolerance by Inhibiting Hif-1α.
Persistent accumulation of unrepaired DNA damage in rat cortical neurons: nuclear organization and ChIP-seq analysis of damaged DNA.
A Dual Role of Caspase-8 in Triggering and Sensing Proliferation-Associated DNA Damage, a Key Determinant of Liver Cancer Development.
Inhibition of Discoidin Domain Receptor 1 Reduces Collagen-mediated Tumorigenicity in Pancreatic Ductal Adenocarcinoma.
Dietary folate levels alter the kinetics and molecular mechanism of prostate cancer recurrence in the CWR22 model.
Sin3a regulates epithelial progenitor cell fate during lung development.
Tracking the origin of simultaneous endometrial and ovarian cancer by next-generation sequencing - a case report.
The helicase domain of Polθ counteracts RPA to promote alt-NHEJ.
Telomeric TERB1-TRF1 interaction is crucial for male meiosis.
Cymbopogon citratus and Camellia sinensis extracts selectively induce apoptosis in cancer cells and reduce growth of lymphoma xenografts in vivo.
Copper induces hepatocyte injury due to the endoplasmic reticulum stress in cultured cells and patients with Wilson disease.
Loss of IκB kinase β promotes myofibroblast transformation and senescence through activation of the ROS-TGFβ autocrine loop.
Sebaceous lipids are essential for water repulsion, protection against UVB-induced apoptosis and ocular integrity in mice.
Beta emitters rhenium-188 and lutetium-177 are equally effective in radioimmunotherapy of HPV-positive experimental cervical cancer.
DNA-PK triggers histone ubiquitination and signaling in response to DNA double-strand breaks produced during the repair of transcription-blocking topoisomerase I lesions.
Neuronal accumulation of unrepaired DNA in a novel specific chromatin domain: structural, molecular and transcriptional characterization.
Controlled DNA double-strand break induction in mice reveals post-damage transcriptome stability.
DICER, DROSHA and DNA damage response RNAs are necessary for the secondary recruitment of DNA damage response factors.
DNA repair foci and late apoptosis/necrosis in peripheral blood lymphocytes of breast cancer patients undergoing radiotherapy.
A novel mouse model for ataxia-telangiectasia with a N-terminal mutation displays a behavioral defect and a low incidence of lymphoma but no increased oxidative burden.
Organization and dynamics of the nonhomologous end-joining machinery during DNA double-strand break repair.
ATM regulation of IL-8 links oxidative stress to cancer cell migration and invasion.
Mammalian polymerase θ promotes alternative NHEJ and suppresses recombination.
Imaging flow cytometry as a sensitive tool to detect low-dose-induced DNA damage by analyzing 53BP1 and γH2AX foci in human lymphocytes.
Phase I Clinical Pharmacology Study of F14512, a New Polyamine-Vectorized Anticancer Drug, in Naturally Occurring Canine Lymphoma.
Translational initiation regulated by ATM in dendritic cells development.
DNA-damage response during mitosis induces whole-chromosome missegregation.
Three-dimensional hydrogel scaffolds facilitate in vitro self-renewal of human skin-derived precursors.
Depletion of the adaptor protein NCK increases UV-induced p53 phosphorylation and promotes apoptosis.
Relative biological efficiency of protons at low and therapeutic doses in induction of 53BP1/γH2AX foci in lymphocytes from umbilical cord blood.
Cyclin A regulates kinetochore microtubules to promote faithful chromosome segregation.
p16(INK) (4a) deficiency promotes DNA hyper-replication and genetic instability in melanocytes.
Retinoic acid derived from the fetal ovary initiates meiosis in mouse germ cells.
Induced dicentric chromosome formation promotes genomic rearrangements and tumorigenesis.
DNA damage response in CD133 + stem/progenitor cells from umbilical cord blood: low level of endogenous foci and high recruitment of 53BP1.
SMC1-mediated intra-S-phase arrest facilitates bocavirus DNA replication.
Mitochondrial topoisomerase I is critical for mitochondrial integrity and cellular energy metabolism.
The Fanconi anaemia components UBE2T and FANCM are functionally linked to nucleotide excision repair.
CK2-mediated hyperphosphorylation of topoisomerase I targets serine 506, enhances topoisomerase I-DNA binding, and increases cellular camptothecin sensitivity.
Three-dimensional imaging reveals the spatial separation of γH2AX-MDC1-53BP1 and RNF8-RNF168-BRCA1-A complexes at ionizing radiation-induced foci.
Non-targeting siRNA induces NPGPx expression to cooperate with exoribonuclease XRN2 for releasing the stress.
Replication-mediated disassociation of replication protein A-XPA complex upon DNA damage: implications for RPA handing off.
E2F7 represses a network of oscillating cell cycle genes to control S-phase progression.
γ-H2AX detection in peripheral blood lymphocytes, splenocytes, bone marrow, xenografts, and skin.
Restoration of G1 chemo/radioresistance and double-strand-break repair proficiency by wild-type but not endonuclease-deficient Artemis.
A novel and simple micro-irradiation technique for creating localized DNA double-strand breaks.
A comparison of BRCA1 nuclear localization with 14 DNA damage response proteins and domains: identification of specific differences between BRCA1 and 53BP1 at DNA damage-induced foci.
Proliferation of aneuploid human cells is limited by a p53-dependent mechanism.
Multimodal assessment of protein functional deficiency supports pathogenicity of BRCA1 p.V1688del.
Temporal regulation of Ig gene diversification revealed by single-cell imaging.
Telomeric DNA mediates de novo PML body formation.
Mammalian Rif1 contributes to replication stress survival and homology-directed repair.
c-Myc-induced aberrant DNA synthesis and activation of DNA damage response in p300 knockdown cells.
Widespread phosphorylation of histone H2AX by species C adenovirus infection requires viral DNA replication.
Continuous elimination of oxidized nucleotides is necessary to prevent rapid onset of cellular senescence.
The direct interaction between 53BP1 and MDC1 is required for the recruitment of 53BP1 to sites of damage.
A population of human brain cells expressing phenotypic markers of more than one lineage can be induced in vitro to differentiate into mesenchymal cells.
Transient inhibition of ATM kinase is sufficient to enhance cellular sensitivity to ionizing radiation.
Rapid telomere motions in live human cells analyzed by highly time-resolved microscopy.
ATM and the Mre11-Rad50-Nbs1 complex respond to nucleoside analogue-induced stalled replication forks and contribute to drug resistance.
Competition effect in DNA damage response.
Quantitative analysis of DNA-damage response factors after sequential ion microirradiation.
The kinetics of p53 activation versus cyclin E accumulation underlies the relationship between the spindle-assembly checkpoint and the postmitotic checkpoint.
ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage.
Calcium-dependent modulation of poly(ADP-ribose) polymerase-1 alters cellular metabolism and DNA repair.
DNA damage responses in progeroid syndromes arise from defective maturation of prelamin A.
H2AX phosphorylation within the G1 phase after UV irradiation depends on nucleotide excision repair and not DNA double-strand breaks.
Differential cellular responses to exogenous DNA in mammalian cells and its effect on oligonucleotide-directed gene modification.
Stalled replication induces p53 accumulation through distinct mechanisms from DNA damage checkpoint pathways.
Histone deacetylase (HDAC) inhibitor LBH589 increases duration of gamma-H2AX foci and confines HDAC4 to the cytoplasm in irradiated non-small cell lung cancer.
Involvement of novel autophosphorylation sites in ATM activation.
Polyploidization increases the sensitivity to DNA-damaging agents in mammalian cells.
Senescence of human fibroblasts after psoralen photoactivation is mediated by ATR kinase and persistent DNA damage foci at telomeres.
Haeger A, Alexander S, Vullings M, Kaiser FMP, Veelken C, Flucke U, Koehl GE, Hirschberg M, Flentje M, Hoffman RM, Geissler EK, Kissler S, Friedl P
The Journal of experimental medicine 2020 Jan 6;217(1)
The Journal of experimental medicine 2020 Jan 6;217(1)
Association of glomerular DNA damage and DNA methylation with one-year eGFR decline in IgA nephropathy.
Hayashi K, Hishikawa A, Hashiguchi A, Azegami T, Yoshimoto N, Nakamichi R, Tokuyama H, Itoh H
Scientific reports 2020 Jan 14;10(1):237
Scientific reports 2020 Jan 14;10(1):237
Randomized, double-blind trial of F14512, a polyamine-vectorized anticancer drug, compared with etoposide phosphate, in dogs with naturally occurring lymphoma.
Boyé P, Floch F, Serres F, Segaoula Z, Hordeaux J, Pascal Q, Coste V, Courapied S, Bouchaert E, Rybicka A, Mazuy C, Marescaux L, Geeraert K, Fournel-Fleury C, Duhamel A, Machuron F, Ferré P, Pétain A, Guilbaud N, Tierny D, Gomes B
Oncotarget 2020 Feb 18;11(7):671-686
Oncotarget 2020 Feb 18;11(7):671-686
Dietary riboflavin deficiency promotes N-nitrosomethylbenzylamine-induced esophageal tumorigenesis in rats by inducing chronic inflammation.
Pan F, Chen Y, He JZ, Long L, Chen Y, Luo HJ, Xu YW, Pang XX, Yang Q, Wang JJ, Xu XE, Wang SH, Li EM, Xu LY
American journal of cancer research 2019;9(11):2469-2481
American journal of cancer research 2019;9(11):2469-2481
CTDP1 regulates breast cancer survival and DNA repair through BRCT-specific interactions with FANCI.
Hu WF, Krieger KL, Lagundžin D, Li X, Cheung RS, Taniguchi T, Johnson KR, Bessho T, Monteiro ANA, Woods NT
Cell death discovery 2019;5:105
Cell death discovery 2019;5:105
Methods to Quantify the NF-κB Pathway During Senescence.
Zhang L, Zhao J, Gurkar A, Niedernhofer LJ, Robbins PD
Methods in molecular biology (Clifton, N.J.) 2019;1896:231-250
Methods in molecular biology (Clifton, N.J.) 2019;1896:231-250
Mouse Models of Accelerated Cellular Senescence.
Yousefzadeh MJ, Melos KI, Angelini L, Burd CE, Robbins PD, Niedernhofer LJ
Methods in molecular biology (Clifton, N.J.) 2019;1896:203-230
Methods in molecular biology (Clifton, N.J.) 2019;1896:203-230
SET Domain-Containing Protein 4 Epigenetically Controls Breast Cancer Stem Cell Quiescence.
Ye S, Ding YF, Jia WH, Liu XL, Feng JY, Zhu Q, Cai SL, Yang YS, Lu QY, Huang XT, Yang JS, Jia SN, Ding GP, Wang YH, Zhou JJ, Chen YD, Yang WJ
Cancer research 2019 Sep 15;79(18):4729-4743
Cancer research 2019 Sep 15;79(18):4729-4743
Anti-Tumor Effects of Wee1 Kinase Inhibitor with Radiotherapy in Human Cervical Cancer.
Lee YY, Cho YJ, Shin SW, Choi C, Ryu JY, Jeon HK, Choi JJ, Hwang JR, Choi CH, Kim TJ, Kim BG, Bae DS, Park W, Lee JW
Scientific reports 2019 Oct 28;9(1):15394
Scientific reports 2019 Oct 28;9(1):15394
Functional Impacts of the BRCA1-mTORC2 Interaction in Breast Cancer.
Krieger KL, Hu WF, Ripperger T, Woods NT
International journal of molecular sciences 2019 Nov 23;20(23)
International journal of molecular sciences 2019 Nov 23;20(23)
BRCA1 Haploinsufficiency Is Masked by RNF168-Mediated Chromatin Ubiquitylation.
Zong D, Adam S, Wang Y, Sasanuma H, Callén E, Murga M, Day A, Kruhlak MJ, Wong N, Munro M, Ray Chaudhuri A, Karim B, Xia B, Takeda S, Johnson N, Durocher D, Nussenzweig A
Molecular cell 2019 Mar 21;73(6):1267-1281.e7
Molecular cell 2019 Mar 21;73(6):1267-1281.e7
DNA Replication Vulnerabilities Render Ovarian Cancer Cells Sensitive to Poly(ADP-Ribose) Glycohydrolase Inhibitors.
Pillay N, Tighe A, Nelson L, Littler S, Coulson-Gilmer C, Bah N, Golder A, Bakker B, Spierings DCJ, James DI, Smith KM, Jordan AM, Morgan RD, Ogilvie DJ, Foijer F, Jackson DA, Taylor SS
Cancer cell 2019 Mar 18;35(3):519-533.e8
Cancer cell 2019 Mar 18;35(3):519-533.e8
p53-TP53-Induced Glycolysis Regulator Mediated Glycolytic Suppression Attenuates DNA Damage and Genomic Instability in Fanconi Anemia Hematopoietic Stem Cells.
Li X, Wu L, Zopp M, Kopelov S, Du W
Stem cells (Dayton, Ohio) 2019 Jul;37(7):937-947
Stem cells (Dayton, Ohio) 2019 Jul;37(7):937-947
PARP inhibitor re‑sensitizes Adriamycin resistant leukemia cells through DNA damage and apoptosis.
Wu J, Xiao S, Yuan M, Li Q, Xiao G, Wu W, Ouyang Y, Huang L, Yao C
Molecular medicine reports 2019 Jan;19(1):75-84
Molecular medicine reports 2019 Jan;19(1):75-84
Reprogramming of Meiotic Chromatin Architecture during Spermatogenesis.
Wang Y, Wang H, Zhang Y, Du Z, Si W, Fan S, Qin D, Wang M, Duan Y, Li L, Jiao Y, Li Y, Wang Q, Shi Q, Wu X, Xie W
Molecular cell 2019 Feb 7;73(3):547-561.e6
Molecular cell 2019 Feb 7;73(3):547-561.e6
A truncating mutation in the autophagy gene UVRAG drives inflammation and tumorigenesis in mice.
Quach C, Song Y, Guo H, Li S, Maazi H, Fung M, Sands N, O'Connell D, Restrepo-Vassalli S, Chai B, Nemecio D, Punj V, Akbari O, Idos GE, Mumenthaler SM, Wu N, Martin SE, Hagiya A, Hicks J, Cui H, Liang C
Nature communications 2019 Dec 12;10(1):5681
Nature communications 2019 Dec 12;10(1):5681
Systematic bromodomain protein screens identify homologous recombination and R-loop suppression pathways involved in genome integrity.
Kim JJ, Lee SY, Gong F, Battenhouse AM, Boutz DR, Bashyal A, Refvik ST, Chiang CM, Xhemalce B, Paull TT, Brodbelt JS, Marcotte EM, Miller KM
Genes & development 2019 Dec 1;33(23-24):1751-1774
Genes & development 2019 Dec 1;33(23-24):1751-1774
The p38α Stress Kinase Suppresses Aneuploidy Tolerance by Inhibiting Hif-1α.
Simões-Sousa S, Littler S, Thompson SL, Minshall P, Whalley H, Bakker B, Belkot K, Moralli D, Bronder D, Tighe A, Spierings DCJ, Bah N, Graham J, Nelson L, Green CM, Foijer F, Townsend PA, Taylor SS
Cell reports 2018 Oct 16;25(3):749-760.e6
Cell reports 2018 Oct 16;25(3):749-760.e6
Persistent accumulation of unrepaired DNA damage in rat cortical neurons: nuclear organization and ChIP-seq analysis of damaged DNA.
Mata-Garrido J, Tapia O, Casafont I, Berciano MT, Cuadrado A, Lafarga M
Acta neuropathologica communications 2018 Jul 26;6(1):68
Acta neuropathologica communications 2018 Jul 26;6(1):68
A Dual Role of Caspase-8 in Triggering and Sensing Proliferation-Associated DNA Damage, a Key Determinant of Liver Cancer Development.
Boege Y, Malehmir M, Healy ME, Bettermann K, Lorentzen A, Vucur M, Ahuja AK, Böhm F, Mertens JC, Shimizu Y, Frick L, Remouchamps C, Mutreja K, Kähne T, Sundaravinayagam D, Wolf MJ, Rehrauer H, Koppe C, Speicher T, Padrissa-Altés S, Maire R, Schattenberg JM, Jeong JS, Liu L, Zwirner S, Boger R, Hüser N, Davis RJ, Müllhaupt B, Moch H, Schulze-Bergkamen H, Clavien PA, Werner S, Borsig L, Luther SA, Jost PJ, Weinlich R, Unger K, Behrens A, Hillert L, Dillon C, Di Virgilio M, Wallach D, Dejardin E, Zender L, Naumann M, Walczak H, Green DR, Lopes M, Lavrik I, Luedde T, Heikenwalder M, Weber A
Cancer cell 2017 Sep 11;32(3):342-359.e10
Cancer cell 2017 Sep 11;32(3):342-359.e10
Inhibition of Discoidin Domain Receptor 1 Reduces Collagen-mediated Tumorigenicity in Pancreatic Ductal Adenocarcinoma.
Aguilera KY, Huang H, Du W, Hagopian MM, Wang Z, Hinz S, Hwang TH, Wang H, Fleming JB, Castrillon DH, Ren X, Ding K, Brekken RA
Molecular cancer therapeutics 2017 Nov;16(11):2473-2485
Molecular cancer therapeutics 2017 Nov;16(11):2473-2485
Dietary folate levels alter the kinetics and molecular mechanism of prostate cancer recurrence in the CWR22 model.
Affronti HC, Long MD, Rosario SR, Gillard BM, Karasik E, Boerlin CS, Pellerite AJ, Foster BA, Attwood K, Pili R, Wilton JH, Campbell MJ, Smiraglia DJ
Oncotarget 2017 Nov 28;8(61):103758-103774
Oncotarget 2017 Nov 28;8(61):103758-103774
Sin3a regulates epithelial progenitor cell fate during lung development.
Yao C, Carraro G, Konda B, Guan X, Mizuno T, Chiba N, Kostelny M, Kurkciyan A, David G, McQualter JL, Stripp BR
Development (Cambridge, England) 2017 Jul 15;144(14):2618-2628
Development (Cambridge, England) 2017 Jul 15;144(14):2618-2628
Tracking the origin of simultaneous endometrial and ovarian cancer by next-generation sequencing - a case report.
Valtcheva N, Lang FM, Noske A, Samartzis EP, Schmidt AM, Bellini E, Fink D, Moch H, Rechsteiner M, Dedes KJ, Wild PJ
BMC cancer 2017 Jan 19;17(1):66
BMC cancer 2017 Jan 19;17(1):66
The helicase domain of Polθ counteracts RPA to promote alt-NHEJ.
Mateos-Gomez PA, Kent T, Deng SK, McDevitt S, Kashkina E, Hoang TM, Pomerantz RT, Sfeir A
Nature structural & molecular biology 2017 Dec;24(12):1116-1123
Nature structural & molecular biology 2017 Dec;24(12):1116-1123
Telomeric TERB1-TRF1 interaction is crucial for male meiosis.
Long J, Huang C, Chen Y, Zhang Y, Shi S, Wu L, Liu Y, Liu C, Wu J, Lei M
Nature structural & molecular biology 2017 Dec;24(12):1073-1080
Nature structural & molecular biology 2017 Dec;24(12):1073-1080
Cymbopogon citratus and Camellia sinensis extracts selectively induce apoptosis in cancer cells and reduce growth of lymphoma xenografts in vivo.
Philion C, Ma D, Ruvinov I, Mansour F, Pignanelli C, Noel M, Saleem A, Arnason J, Rodrigues M, Singh I, Ropat J, Pandey S
Oncotarget 2017 Dec 19;8(67):110756-110773
Oncotarget 2017 Dec 19;8(67):110756-110773
Copper induces hepatocyte injury due to the endoplasmic reticulum stress in cultured cells and patients with Wilson disease.
Oe S, Miyagawa K, Honma Y, Harada M
Experimental cell research 2016 Sep 10;347(1):192-200
Experimental cell research 2016 Sep 10;347(1):192-200
Loss of IκB kinase β promotes myofibroblast transformation and senescence through activation of the ROS-TGFβ autocrine loop.
Chen L, Peng Z, Meng Q, Mongan M, Wang J, Sartor M, Chen J, Niu L, Medvedovic M, Kao W, Xia Y
Protein & cell 2016 May;7(5):338-50
Protein & cell 2016 May;7(5):338-50
Sebaceous lipids are essential for water repulsion, protection against UVB-induced apoptosis and ocular integrity in mice.
Dahlhoff M, Camera E, Schäfer M, Emrich D, Riethmacher D, Foster A, Paus R, Schneider MR
Development (Cambridge, England) 2016 May 15;143(10):1823-31
Development (Cambridge, England) 2016 May 15;143(10):1823-31
Beta emitters rhenium-188 and lutetium-177 are equally effective in radioimmunotherapy of HPV-positive experimental cervical cancer.
Phaeton R, Jiang Z, Revskaya E, Fisher DR, Goldberg GL, Dadachova E
Cancer medicine 2016 Jan;5(1):9-16
Cancer medicine 2016 Jan;5(1):9-16
DNA-PK triggers histone ubiquitination and signaling in response to DNA double-strand breaks produced during the repair of transcription-blocking topoisomerase I lesions.
Cristini A, Park JH, Capranico G, Legube G, Favre G, Sordet O
Nucleic acids research 2016 Feb 18;44(3):1161-78
Nucleic acids research 2016 Feb 18;44(3):1161-78
Neuronal accumulation of unrepaired DNA in a novel specific chromatin domain: structural, molecular and transcriptional characterization.
Mata-Garrido J, Casafont I, Tapia O, Berciano MT, Lafarga M
Acta neuropathologica communications 2016 Apr 22;4:41
Acta neuropathologica communications 2016 Apr 22;4:41
Controlled DNA double-strand break induction in mice reveals post-damage transcriptome stability.
Kim J, Sturgill D, Tran AD, Sinclair DA, Oberdoerffer P
Nucleic acids research 2016 Apr 20;44(7):e64
Nucleic acids research 2016 Apr 20;44(7):e64
DICER, DROSHA and DNA damage response RNAs are necessary for the secondary recruitment of DNA damage response factors.
Francia S, Cabrini M, Matti V, Oldani A, d'Adda di Fagagna F
Journal of cell science 2016 Apr 1;129(7):1468-76
Journal of cell science 2016 Apr 1;129(7):1468-76
DNA repair foci and late apoptosis/necrosis in peripheral blood lymphocytes of breast cancer patients undergoing radiotherapy.
Marková E, Somsedíková A, Vasilyev S, Pobijaková M, Lacková A, Lukačko P, Belyaev I
International journal of radiation biology 2015;91(12):934-45
International journal of radiation biology 2015;91(12):934-45
A novel mouse model for ataxia-telangiectasia with a N-terminal mutation displays a behavioral defect and a low incidence of lymphoma but no increased oxidative burden.
Campbell A, Krupp B, Bushman J, Noble M, Pröschel C, Mayer-Pröschel M
Human molecular genetics 2015 Nov 15;24(22):6331-49
Human molecular genetics 2015 Nov 15;24(22):6331-49
Organization and dynamics of the nonhomologous end-joining machinery during DNA double-strand break repair.
Reid DA, Keegan S, Leo-Macias A, Watanabe G, Strande NT, Chang HH, Oksuz BA, Fenyo D, Lieber MR, Ramsden DA, Rothenberg E
Proceedings of the National Academy of Sciences of the United States of America 2015 May 19;112(20):E2575-84
Proceedings of the National Academy of Sciences of the United States of America 2015 May 19;112(20):E2575-84
ATM regulation of IL-8 links oxidative stress to cancer cell migration and invasion.
Chen WT, Ebelt ND, Stracker TH, Xhemalce B, Van Den Berg CL, Miller KM
eLife 2015 Jun 1;4
eLife 2015 Jun 1;4
Mammalian polymerase θ promotes alternative NHEJ and suppresses recombination.
Mateos-Gomez PA, Gong F, Nair N, Miller KM, Lazzerini-Denchi E, Sfeir A
Nature 2015 Feb 12;518(7538):254-7
Nature 2015 Feb 12;518(7538):254-7
Imaging flow cytometry as a sensitive tool to detect low-dose-induced DNA damage by analyzing 53BP1 and γH2AX foci in human lymphocytes.
Durdik M, Kosik P, Gursky J, Vokalova L, Markova E, Belyaev I
Cytometry. Part A : the journal of the International Society for Analytical Cytology 2015 Dec;87(12):1070-8
Cytometry. Part A : the journal of the International Society for Analytical Cytology 2015 Dec;87(12):1070-8
Phase I Clinical Pharmacology Study of F14512, a New Polyamine-Vectorized Anticancer Drug, in Naturally Occurring Canine Lymphoma.
Tierny D, Serres F, Segaoula Z, Bemelmans I, Bouchaert E, Pétain A, Brel V, Couffin S, Marchal T, Nguyen L, Thuru X, Ferré P, Guilbaud N, Gomes B
Clinical cancer research : an official journal of the American Association for Cancer Research 2015 Dec 1;21(23):5314-23
Clinical cancer research : an official journal of the American Association for Cancer Research 2015 Dec 1;21(23):5314-23
Translational initiation regulated by ATM in dendritic cells development.
So EY, Ouchi T
Cell death & disease 2014 Sep 11;5:e1418
Cell death & disease 2014 Sep 11;5:e1418
DNA-damage response during mitosis induces whole-chromosome missegregation.
Bakhoum SF, Kabeche L, Murnane JP, Zaki BI, Compton DA
Cancer discovery 2014 Nov;4(11):1281-9
Cancer discovery 2014 Nov;4(11):1281-9
Three-dimensional hydrogel scaffolds facilitate in vitro self-renewal of human skin-derived precursors.
Wang X, Liu S, Zhao Q, Li N, Zhang H, Zhang X, Lei X, Zhao H, Deng Z, Qiao J, Cao Y, Ning L, Liu S, Duan E
Acta biomaterialia 2014 Jul;10(7):3177-87
Acta biomaterialia 2014 Jul;10(7):3177-87
Depletion of the adaptor protein NCK increases UV-induced p53 phosphorylation and promotes apoptosis.
Errington TM, Macara IG
PloS one 2013;8(9):e76204
PloS one 2013;8(9):e76204
Relative biological efficiency of protons at low and therapeutic doses in induction of 53BP1/γH2AX foci in lymphocytes from umbilical cord blood.
Sorokina S, Markova E, Gursky J, Dobrovodsky J, Belyaev I
International journal of radiation biology 2013 Sep;89(9):716-23
International journal of radiation biology 2013 Sep;89(9):716-23
Cyclin A regulates kinetochore microtubules to promote faithful chromosome segregation.
Kabeche L, Compton DA
Nature 2013 Oct 3;502(7469):110-3
Nature 2013 Oct 3;502(7469):110-3
p16(INK) (4a) deficiency promotes DNA hyper-replication and genetic instability in melanocytes.
Fung C, Pupo GM, Scolyer RA, Kefford RF, Rizos H
Pigment cell & melanoma research 2013 Mar;26(2):236-46
Pigment cell & melanoma research 2013 Mar;26(2):236-46
Retinoic acid derived from the fetal ovary initiates meiosis in mouse germ cells.
Mu X, Wen J, Guo M, Wang J, Li G, Wang Z, Wang Y, Teng Z, Cui Y, Xia G
Journal of cellular physiology 2013 Mar;228(3):627-39
Journal of cellular physiology 2013 Mar;228(3):627-39
Induced dicentric chromosome formation promotes genomic rearrangements and tumorigenesis.
Gascoigne KE, Cheeseman IM
Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology 2013 Jul;21(4):407-18
Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology 2013 Jul;21(4):407-18
DNA damage response in CD133 + stem/progenitor cells from umbilical cord blood: low level of endogenous foci and high recruitment of 53BP1.
Vasilyev SA, Kubes M, Markova E, Belyaev I
International journal of radiation biology 2013 Apr;89(4):301-9
International journal of radiation biology 2013 Apr;89(4):301-9
SMC1-mediated intra-S-phase arrest facilitates bocavirus DNA replication.
Luo Y, Deng X, Cheng F, Li Y, Qiu J
Journal of virology 2013 Apr;87(7):4017-32
Journal of virology 2013 Apr;87(7):4017-32
Mitochondrial topoisomerase I is critical for mitochondrial integrity and cellular energy metabolism.
Douarre C, Sourbier C, Dalla Rosa I, Brata Das B, Redon CE, Zhang H, Neckers L, Pommier Y
PloS one 2012;7(7):e41094
PloS one 2012;7(7):e41094
The Fanconi anaemia components UBE2T and FANCM are functionally linked to nucleotide excision repair.
Kelsall IR, Langenick J, MacKay C, Patel KJ, Alpi AF
PloS one 2012;7(5):e36970
PloS one 2012;7(5):e36970
CK2-mediated hyperphosphorylation of topoisomerase I targets serine 506, enhances topoisomerase I-DNA binding, and increases cellular camptothecin sensitivity.
Bandyopadhyay K, Li P, Gjerset RA
PloS one 2012;7(11):e50427
PloS one 2012;7(11):e50427
Three-dimensional imaging reveals the spatial separation of γH2AX-MDC1-53BP1 and RNF8-RNF168-BRCA1-A complexes at ionizing radiation-induced foci.
Mok MT, Henderson BR
Radiotherapy and oncology : journal of the European Society for Therapeutic Radiology and Oncology 2012 Jun;103(3):415-20
Radiotherapy and oncology : journal of the European Society for Therapeutic Radiology and Oncology 2012 Jun;103(3):415-20
Non-targeting siRNA induces NPGPx expression to cooperate with exoribonuclease XRN2 for releasing the stress.
Wei PC, Lo WT, Su MI, Shew JY, Lee WH
Nucleic acids research 2012 Jan;40(1):323-32
Nucleic acids research 2012 Jan;40(1):323-32
Replication-mediated disassociation of replication protein A-XPA complex upon DNA damage: implications for RPA handing off.
Jiang G, Zou Y, Wu X
Cell biology international 2012 Aug 1;36(8):713-20
Cell biology international 2012 Aug 1;36(8):713-20
E2F7 represses a network of oscillating cell cycle genes to control S-phase progression.
Westendorp B, Mokry M, Groot Koerkamp MJ, Holstege FC, Cuppen E, de Bruin A
Nucleic acids research 2012 Apr;40(8):3511-23
Nucleic acids research 2012 Apr;40(8):3511-23
γ-H2AX detection in peripheral blood lymphocytes, splenocytes, bone marrow, xenografts, and skin.
Redon CE, Nakamura AJ, Sordet O, Dickey JS, Gouliaeva K, Tabb B, Lawrence S, Kinders RJ, Bonner WM, Sedelnikova OA
Methods in molecular biology (Clifton, N.J.) 2011;682:249-70
Methods in molecular biology (Clifton, N.J.) 2011;682:249-70
Restoration of G1 chemo/radioresistance and double-strand-break repair proficiency by wild-type but not endonuclease-deficient Artemis.
Mohapatra S, Kawahara M, Khan IS, Yannone SM, Povirk LF
Nucleic acids research 2011 Aug;39(15):6500-10
Nucleic acids research 2011 Aug;39(15):6500-10
A novel and simple micro-irradiation technique for creating localized DNA double-strand breaks.
Suzuki K, Yamauchi M, Oka Y, Suzuki M, Yamashita S
Nucleic acids research 2010 Jul;38(12):e129
Nucleic acids research 2010 Jul;38(12):e129
A comparison of BRCA1 nuclear localization with 14 DNA damage response proteins and domains: identification of specific differences between BRCA1 and 53BP1 at DNA damage-induced foci.
Mok MT, Henderson BR
Cellular signalling 2010 Jan;22(1):47-56
Cellular signalling 2010 Jan;22(1):47-56
Proliferation of aneuploid human cells is limited by a p53-dependent mechanism.
Thompson SL, Compton DA
The Journal of cell biology 2010 Feb 8;188(3):369-81
The Journal of cell biology 2010 Feb 8;188(3):369-81
Multimodal assessment of protein functional deficiency supports pathogenicity of BRCA1 p.V1688del.
De Nicolo A, Parisini E, Zhong Q, Dalla Palma M, Stoeckert KA, Domchek SM, Nathanson KL, Caligo MA, Vidal M, Cusick ME, Garber JE
Cancer research 2009 Sep 1;69(17):7030-7
Cancer research 2009 Sep 1;69(17):7030-7
Temporal regulation of Ig gene diversification revealed by single-cell imaging.
Ordinario EC, Yabuki M, Larson RP, Maizels N
Journal of immunology (Baltimore, Md. : 1950) 2009 Oct 1;183(7):4545-53
Journal of immunology (Baltimore, Md. : 1950) 2009 Oct 1;183(7):4545-53
Telomeric DNA mediates de novo PML body formation.
Brouwer AK, Schimmel J, Wiegant JC, Vertegaal AC, Tanke HJ, Dirks RW
Molecular biology of the cell 2009 Nov;20(22):4804-15
Molecular biology of the cell 2009 Nov;20(22):4804-15
Mammalian Rif1 contributes to replication stress survival and homology-directed repair.
Buonomo SB, Wu Y, Ferguson D, de Lange T
The Journal of cell biology 2009 Nov 2;187(3):385-98
The Journal of cell biology 2009 Nov 2;187(3):385-98
c-Myc-induced aberrant DNA synthesis and activation of DNA damage response in p300 knockdown cells.
Sankar N, Kadeppagari RK, Thimmapaya B
The Journal of biological chemistry 2009 May 29;284(22):15193-205
The Journal of biological chemistry 2009 May 29;284(22):15193-205
Widespread phosphorylation of histone H2AX by species C adenovirus infection requires viral DNA replication.
Nichols GJ, Schaack J, Ornelles DA
Journal of virology 2009 Jun;83(12):5987-98
Journal of virology 2009 Jun;83(12):5987-98
Continuous elimination of oxidized nucleotides is necessary to prevent rapid onset of cellular senescence.
Rai P, Onder TT, Young JJ, McFaline JL, Pang B, Dedon PC, Weinberg RA
Proceedings of the National Academy of Sciences of the United States of America 2009 Jan 6;106(1):169-74
Proceedings of the National Academy of Sciences of the United States of America 2009 Jan 6;106(1):169-74
The direct interaction between 53BP1 and MDC1 is required for the recruitment of 53BP1 to sites of damage.
Eliezer Y, Argaman L, Rhie A, Doherty AJ, Goldberg M
The Journal of biological chemistry 2009 Jan 2;284(1):426-35
The Journal of biological chemistry 2009 Jan 2;284(1):426-35
A population of human brain cells expressing phenotypic markers of more than one lineage can be induced in vitro to differentiate into mesenchymal cells.
Rieske P, Augelli BJ, Stawski R, Gaughan J, Azizi SA, Krynska B
Experimental cell research 2009 Feb 1;315(3):462-73
Experimental cell research 2009 Feb 1;315(3):462-73
Transient inhibition of ATM kinase is sufficient to enhance cellular sensitivity to ionizing radiation.
Rainey MD, Charlton ME, Stanton RV, Kastan MB
Cancer research 2008 Sep 15;68(18):7466-74
Cancer research 2008 Sep 15;68(18):7466-74
Rapid telomere motions in live human cells analyzed by highly time-resolved microscopy.
Wang X, Kam Z, Carlton PM, Xu L, Sedat JW, Blackburn EH
Epigenetics & chromatin 2008 Oct 27;1(1):4
Epigenetics & chromatin 2008 Oct 27;1(1):4
ATM and the Mre11-Rad50-Nbs1 complex respond to nucleoside analogue-induced stalled replication forks and contribute to drug resistance.
Ewald B, Sampath D, Plunkett W
Cancer research 2008 Oct 1;68(19):7947-55
Cancer research 2008 Oct 1;68(19):7947-55
Competition effect in DNA damage response.
Greubel C, Hable V, Drexler GA, Hauptner A, Dietzel S, Strickfaden H, Baur I, Krücken R, Cremer T, Dollinger G, Friedl AA
Radiation and environmental biophysics 2008 Nov;47(4):423-9
Radiation and environmental biophysics 2008 Nov;47(4):423-9
Quantitative analysis of DNA-damage response factors after sequential ion microirradiation.
Greubel C, Hable V, Drexler GA, Hauptner A, Dietzel S, Strickfaden H, Baur I, Krücken R, Cremer T, Friedl AA, Dollinger G
Radiation and environmental biophysics 2008 Nov;47(4):415-22
Radiation and environmental biophysics 2008 Nov;47(4):415-22
The kinetics of p53 activation versus cyclin E accumulation underlies the relationship between the spindle-assembly checkpoint and the postmitotic checkpoint.
Chan YW, On KF, Chan WM, Wong W, Siu HO, Hau PM, Poon RY
The Journal of biological chemistry 2008 Jun 6;283(23):15716-23
The Journal of biological chemistry 2008 Jun 6;283(23):15716-23
ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage.
Matsuoka S, Ballif BA, Smogorzewska A, McDonald ER 3rd, Hurov KE, Luo J, Bakalarski CE, Zhao Z, Solimini N, Lerenthal Y, Shiloh Y, Gygi SP, Elledge SJ
Science (New York, N.Y.) 2007 May 25;316(5828):1160-6
Science (New York, N.Y.) 2007 May 25;316(5828):1160-6
Calcium-dependent modulation of poly(ADP-ribose) polymerase-1 alters cellular metabolism and DNA repair.
Bentle MS, Reinicke KE, Bey EA, Spitz DR, Boothman DA
The Journal of biological chemistry 2006 Nov 3;281(44):33684-96
The Journal of biological chemistry 2006 Nov 3;281(44):33684-96
DNA damage responses in progeroid syndromes arise from defective maturation of prelamin A.
Liu Y, Rusinol A, Sinensky M, Wang Y, Zou Y
Journal of cell science 2006 Nov 15;119(Pt 22):4644-9
Journal of cell science 2006 Nov 15;119(Pt 22):4644-9
H2AX phosphorylation within the G1 phase after UV irradiation depends on nucleotide excision repair and not DNA double-strand breaks.
Marti TM, Hefner E, Feeney L, Natale V, Cleaver JE
Proceedings of the National Academy of Sciences of the United States of America 2006 Jun 27;103(26):9891-6
Proceedings of the National Academy of Sciences of the United States of America 2006 Jun 27;103(26):9891-6
Differential cellular responses to exogenous DNA in mammalian cells and its effect on oligonucleotide-directed gene modification.
Igoucheva O, Alexeev V, Yoon K
Gene therapy 2006 Feb;13(3):266-75
Gene therapy 2006 Feb;13(3):266-75
Stalled replication induces p53 accumulation through distinct mechanisms from DNA damage checkpoint pathways.
Ho CC, Siu WY, Lau A, Chan WM, Arooz T, Poon RY
Cancer research 2006 Feb 15;66(4):2233-41
Cancer research 2006 Feb 15;66(4):2233-41
Histone deacetylase (HDAC) inhibitor LBH589 increases duration of gamma-H2AX foci and confines HDAC4 to the cytoplasm in irradiated non-small cell lung cancer.
Geng L, Cuneo KC, Fu A, Tu T, Atadja PW, Hallahan DE
Cancer research 2006 Dec 1;66(23):11298-304
Cancer research 2006 Dec 1;66(23):11298-304
Involvement of novel autophosphorylation sites in ATM activation.
Kozlov SV, Graham ME, Peng C, Chen P, Robinson PJ, Lavin MF
The EMBO journal 2006 Aug 9;25(15):3504-14
The EMBO journal 2006 Aug 9;25(15):3504-14
Polyploidization increases the sensitivity to DNA-damaging agents in mammalian cells.
Hau PM, Siu WY, Wong N, Lai PB, Poon RY
FEBS letters 2006 Aug 21;580(19):4727-36
FEBS letters 2006 Aug 21;580(19):4727-36
Senescence of human fibroblasts after psoralen photoactivation is mediated by ATR kinase and persistent DNA damage foci at telomeres.
Hovest MG, Brüggenolte N, Hosseini KS, Krieg T, Herrmann G
Molecular biology of the cell 2006 Apr;17(4):1758-67
Molecular biology of the cell 2006 Apr;17(4):1758-67
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Supportive validation
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- Western Blot: gamma H2AX [p Ser139] Antibody [NB100-384] - Detection of Human and Mouse gamma H2AX by Western Blot. Samples: Nuclear extract (50 ug) from human HEK293, human melanoma (G361), mouse wildtype embryonic fibroblasts (+/+) or mouse H2AX knockout embryonic fibroblasts (-/-). Antibody: Affinity purified rabbit anti-gamma H2AX antibody NB100-384 used at 0.1 ug/ml. Detection: Chemiluminescence with 30 second exposure. (NCS, neocarzinostatin - 200 ng/ml, 30 min).
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- Simple Western: gamma H2AX [p Ser139] Antibody [NB100-384] - Simple Western lane view shows a specific band for gamma H2AX in 0.2 mg/ml of Jurkat lysate(s). This experiment was performed under reducing conditions using the 12 - 230 kDa separation system.
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- Simple Western: gamma H2AX [p Ser139] Antibody [NB100-384] - Electropherogram image(s) of corresponding Simple Western lane view. Gamma H2AX antibody was used at 5 ug/ml dilution on Jurkat lysate(s).
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- Western Blot: gamma H2AX [p Ser139] Antibody [NB100-384] - Samples: Nuclear extract from HeLa cells treated with 100 uM EPE for 4 hours (+) or mock treated (-). Antibody: Affinity purified rabbit anti-gamma-H2AX antibody used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
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- Western Blot: gamma H2AX [p Ser139] Antibody [NB100-384] - Detection of human gamma-H2AX by western blot. Samples: Whole cell lysate (50 ug) from Jurkat cells treated with 100 uM EPE for 4 hours (+) or mock treated (-). Antibody: Affinity purified rabbit anti-gamma-H2AX antibody NB100-384 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.
Supportive validation
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- Immunohistochemistry: gamma H2AX [p Ser139] Antibody [NB100-384] - Samples: Neocarzinostatin treated asynchronous HeLa cells (left) and untreated asynchronous HeLa cells (right) . Antibody: Affinity purified rabbit anti-gamma-H2AX used at a dilution of 1:5,000 (0.2ug/ml). Detection: Red fluorescent Anti-rabbit IgG-DyLight 594 conjugated used at a dilution of 1:100.
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- Immunohistochemistry: gamma H2AX [p Ser139] Antibody [NB100-384] - Sample: FFPE section of human testicular seminoma. Antibody: Affinity purified rabbit anti- gamma-H2AX used at a dilution of 1:1,000 (1 ug/ml). Detection: DAB
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- Immunohistochemistry: gamma H2AX [p Ser139] Antibody [NB100-384] - Detection of human gamma-H2AX by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti- gamma-H2AX (NB100-384). Detection: DAB.
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- Immunohistochemistry: gamma H2AX [p Ser139] Antibody [NB100-384] - Detection of mouse gamma-H2AX by immunohistochemistry. Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti- gamma-H2AX (NB100-384). Detection: DAB.
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- Flow Cytometry: gamma H2AX [p Ser139] Antibody [NB100-384] - Analysis of gamma-H2AX in EPE Treated Jurkat Cells. Cells were treated for 3 hrs in 5ug/ml etoposide, fixed in 1.5% PFA, and permeabilized in 90% Methanol. 1 million cells were stained with 0.5 ug anti-KLH or anti-H2AX NB100-384 and secondary FITC-conjugated goat anti-rabbit (in a 150ul reaction). Black- etosposide treated, anti-KLH; Red- untreated, anti-gamma H2AX; Blue- etoposide treated , anti-gamma H2AX.
