- GTX628789 - GeneTex H2AX antibody

Submitted references PEGylated liposome-encapsulated rhenium-188 radiopharmaceutical inhibits proliferation and epithelial-mesenchymal transition of human head and neck cancer cells in vivo with repeated therapy.

Chang CY, Chen CC, Lin LT, Chang CH, Chen LC, Wang HE, Lee TW, Lee YJ
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Mycolactone cytotoxicity in Schwann cells could explain nerve damage in Buruli ulcer.

En J, Kitamoto S, Kawashima A, Yonezawa S, Kishi Y, Ishii N, Goto M
PLoS neglected tropical diseases 2017 Aug;11(8):e0005834

A Novel Chemotherapeutic Agent to Treat Tumors with DNA Mismatch Repair Deficiencies.

Zhang Y, Fox JT, Park YU, Elliott G, Rai G, Cai M, Sakamuru S, Huang R, Xia M, Lee K, Jeon MH, Mathew BP, Park HD, Edelmann W, Park CY, Hong SY, Maloney D, Myung K
Cancer research 2016 Jul 15;76(14):4183-91

Fanconi anemia cells with unrepaired DNA damage activate components of the checkpoint recovery process.

Rodríguez A, Torres L, Juárez U, Sosa D, Azpeitia E, García-de Teresa B, Cortés E, Ortíz R, Salazar AM, Ostrosky-Wegman P, Mendoza L, Frías S
Theoretical biology & medical modelling 2015 Sep 18;12:19

Cucurbitacin B induced ATM-mediated DNA damage causes G2/M cell cycle arrest in a ROS-dependent manner.

Guo J, Wu G, Bao J, Hao W, Lu J, Chen X
PloS one 2014;9(2):e88140

Mutant IDH1-driven cellular transformation increases RAD51-mediated homologous recombination and temozolomide resistance.

Ohba S, Mukherjee J, See WL, Pieper RO
Cancer research 2014 Sep 1;74(17):4836-44

Effects of DSP4 on the noradrenergic phenotypes and its potential molecular mechanisms in SH-SY5Y cells.

Wang Y, Musich PR, Serrano MA, Zou Y, Zhang J, Zhu MY
Neurotoxicity research 2014 Feb;25(2):193-207

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Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Histone H2A.X (phospho S139) antibody [GT2311] detects H2AFX protein by western blot analysis.A. 30 ?g HCT116 whole cell lysate/extract (untreated)B. 30 ?g HCT116 whole cell lysate/extract (30 ?M cisplatin treatment for 24hr)12% SDS-PAGEHistone H2A.X (phospho S139) antibody [GT2311] (GTX628789) dilution: 1:1000 The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Histone H2A.X (phospho S139) antibody [GT2311] detects H2AFX protein by western blot analysis.A. 30 ?g NIH-3T3 whole cell lysate/extract (untreated)B. 30 ?g NIH-3T3 whole cell lysate/extract (30?M cisplatin treatment for 24hr)15% SDS-PAGEHistone H2A.X (phospho S139) antibody [GT2311] (GTX628789) dilution: 1:1000 The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Histone H2A.X (phospho S139) antibody [GT2311] detects Histone H2A.X (phospho S139) [GT2311] protein by western blot analysis. Un-treated (-) and treated (+, 30 ?M Cisplatin treatment for 24 hrs) PC-12 whole cell extracts (30 ?g) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H2A.X (phospho S139) antibody [GT2311] (GTX628789) diluted by 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Histone H2A.XS139ph (phospho Ser139) antibody detects Histone H2A.XS139ph (phospho Ser139) protein by western blot analysis. Un-treated (-) and treated (+, 50 J/m2 UV treatment) U2OS whole cell extracts (16 ?g) were separated by 12%-15% SDS-PAGE, and the membrane was blotted with Histone H2A.XS139ph (phospho Ser139) antibody (GTX628789) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Histone H2A.X (phospho Ser139) antibody detects H2AFX protein at nuclear by confocal immunofluorescent analysis. Sample: 10£gM Cisplatin treated (right) or untreated (left) HeLa cells were fixed in 4% paraformaldehyde for 15 min. Red: H2A.X protein stained by Histone H2A.X (phospho Ser139) antibody (GTX628789) diluted at 1:500. Green: alpha Tubulin antibody (GTX102078) diluted at 1:1000. Blue: Hoechst 33342 staining. [Images captured by Olympus FV1000 Confocal Laser Scanning Microscope]

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Histone H2A.X antibody detects H2AFX protein at nuclear by immunofluorescent analysis. Sample: 10£gM Cisplatin treated (right) or untreated (left) HeLa cells were fixed in 4% paraformaldehyde for 15 min. Green: H2AFX protein stained by Histone H2A.Xantibody (GTX628789) diluted at 1:500. Blue: Hoechst 33342 staining.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Histone H2A.X (phospho S139) antibody immunoprecipitates histone H2A.X (phospho S139) protein in IP experiments. IP Sample: 500 £gg HCT116 with CPT 30 £gM treatment 24 hr whole cell lysate/extract A. 30 £gg HCT116 whole with CPT 30 uM treatment cell lysate/extract B. Control with 2 £gg of preimmune mouse IgG C. Immunoprecipitation of histone H2A.X (phospho S139) protein by 2 £gg histone H2A.X (phospho S139) antibody (GTX628789) 15% SDS-PAGE The immunoprecipitated histone H2A.X (phospho S139) protein was detected by Human histone H2A.X (phospho S139) antibody (GTX628789) diluted at 1:1000. EasyBlot anti-mouse IgG (GTX221667-01) was used as a secondary reagent.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Histone H2A.XS139ph (phospho Ser139) antibody [GT2311] detects Histone H2A.XS139ph (phospho Ser139) protein at nucleus on mouse testis by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. Histone H2A.XS139ph (phospho Ser139) antibody [GT2311] (GTX628789) dilution: 1:500.

GTX628789

Antibody data