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Western blot
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- Antibody Data
- Antigen structure
- References [7]
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
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- Product number
- GTX80694 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX80694, RRID:AB_625405
- Product name
- Histone H2A.XS139ph (phospho Ser139) antibody [3F2]
- Antibody type
- Monoclonal
- Reactivity
- Human, Mouse, Bovine
- Host
- Mouse
Submitted references CYT-Rx20 inhibits ovarian cancer cells in vitro and in vivo through oxidative stress-induced DNA damage and cell apoptosis.
Synthetic β-nitrostyrene derivative CYT-Rx20 as inhibitor of oral cancer cell proliferation and tumor growth through glutathione suppression and reactive oxygen species induction.
Curcumin causes DNA damage and affects associated protein expression in HeLa human cervical cancer cells.
Direct activation of ATM by resveratrol under oxidizing conditions.
Histone deacetylase inhibitors selectively target homology dependent DNA repair defective cells and elevate non-homologous endjoining activity.
High-throughput genotoxicity assay identifies antioxidants as inducers of DNA damage response and cell death.
Induction of reactive oxygen species by human T-cell leukemia virus type 1 tax correlates with DNA damage and expression of cellular senescence marker.
Wang YY, Chen YK, Hu SC, Hsu YL, Tsai CH, Chi TC, Huang WL, Hsieh PW, Yuan SF
Cancer chemotherapy and pharmacology 2017 Jun;79(6):1129-1140
Cancer chemotherapy and pharmacology 2017 Jun;79(6):1129-1140
Synthetic β-nitrostyrene derivative CYT-Rx20 as inhibitor of oral cancer cell proliferation and tumor growth through glutathione suppression and reactive oxygen species induction.
Wang YY, Chen YK, Hsu YL, Chiu WC, Tsai CH, Hu SC, Hsieh PW, Yuan SF
Head & neck 2017 Jun;39(6):1055-1064
Head & neck 2017 Jun;39(6):1055-1064
Curcumin causes DNA damage and affects associated protein expression in HeLa human cervical cancer cells.
Shang HS, Chang CH, Chou YR, Yeh MY, Au MK, Lu HF, Chu YL, Chou HM, Chou HC, Shih YL, Chung JG
Oncology reports 2016 Oct;36(4):2207-15
Oncology reports 2016 Oct;36(4):2207-15
Direct activation of ATM by resveratrol under oxidizing conditions.
Lee JH, Guo Z, Myler LR, Zheng S, Paull TT
PloS one 2014;9(6):e97969
PloS one 2014;9(6):e97969
Histone deacetylase inhibitors selectively target homology dependent DNA repair defective cells and elevate non-homologous endjoining activity.
Smith S, Fox J, Mejia M, Ruangpradit W, Saberi A, Kim S, Choi Y, Oh S, Wang Y, Choi K, Li L, Hendrickson EA, Takeda S, Muller M, Myung K
PloS one 2014;9(1):e87203
PloS one 2014;9(1):e87203
High-throughput genotoxicity assay identifies antioxidants as inducers of DNA damage response and cell death.
Fox JT, Sakamuru S, Huang R, Teneva N, Simmons SO, Xia M, Tice RR, Austin CP, Myung K
Proceedings of the National Academy of Sciences of the United States of America 2012 Apr 3;109(14):5423-8
Proceedings of the National Academy of Sciences of the United States of America 2012 Apr 3;109(14):5423-8
Induction of reactive oxygen species by human T-cell leukemia virus type 1 tax correlates with DNA damage and expression of cellular senescence marker.
Kinjo T, Ham-Terhune J, Peloponese JM Jr, Jeang KT
Journal of virology 2010 May;84(10):5431-7
Journal of virology 2010 May;84(10):5431-7
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Western blot detection of phosphorylated H2AX in gamma irradiated HeLA cell lysate. Lane 1: Unphosphorylated H2AX control. Lane 2: Phosphorylated H2AX
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Western blot analysis of Phospho-H2AX pSer140 (GTX80694) at a concentration of 1 ug/ml on Jurkat cell untreated (Lane 1) and Jurkat cell stimulated with staurosporine (Lane 2) followed by HRP conjugated goat anti-mouse lgG (H+L) secondary antibody.
- Validation comment
- WB
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of phosphorylated H2AX in control or G2/M arrested cells.
