Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- 103-PA14 - Provider product page
- Provider
- ReliaTech GmbH
- Product name
- VE-Statin/EGFL7
- Antibody type
- Polyclonal
- Antigen
- Recombinant mouse EGFL7
- Description
- antibody Protein-A purified from serum
- Reactivity
- Mouse
- Host
- Rabbit
- Antibody clone number
- Rabbit IG
- Vial size
- 200 µl
- Storage
- Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
- Handling
- Restore in sterile water to a concentration of 0.1-1.0 mg/ml. The antibody solution should be gently mixed before use.
Submitted references EGFL7 enhances surface expression of integrin α5β1 to promote angiogenesis in malignant brain tumors.
Dudvarski Stanković N, Bicker F, Keller S, Jones DT, Harter PN, Kienzle A, Gillmann C, Arnold P, Golebiewska A, Keunen O, Giese A, von Deimling A, Bäuerle T, Niclou SP, Mittelbronn M, Ye W, Pfister SM, Schmidt MHH
EMBO molecular medicine 2018 Sep;10(9)
EMBO molecular medicine 2018 Sep;10(9)
No comments: Submit comment
Supportive validation
- Submitted by
- ReliaTech GmbH (provider)
- Main image
- Experimental details
- Western analysis of recombinant human and mouse EGFL7. There is no cross reactivity visible with human EGFL-7.
- Sample type
- Purified recombinant proteins
Supportive validation
- Submitted by
- ReliaTech GmbH (provider)
- Main image
- Experimental details
- Immunohistochemical staining (ICC) of human or mouse EGFL7 constructs overexpressed in HEK293T cells.
Supportive validation
- Submitted by
- ReliaTech GmbH (provider)
- Main image
- Experimental details
- Immunoprecipitation of human and mouse EGFL7 constructs with anti-mouse EGFL7 (C terminus) and subsequent Western analysis with anti-V5 antibodies. Samples were loaded in 15% SDS-polyacrylamide gel under reducing conditions. All experiments were performed by Dr. Frank Bicker from the research group „Molecular Signal Transduction“ (Prof. Dr. Mirko H Schmidt), Institute of Microscopic Anatomy and Neurobiology, University Medical Center of Johannes Gutenberg University Mainz, Germany.
- Sample type
- Purified recombinant proteins