AP7650b

antibody from Abgent

Targeting: SESN1 PA26, SEST1

Western blot (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

AP7650b

Provider

Abgent

Proper citation

Abgent Cat#AP7650b, RRID:AB_2185474

Product name

Sestrin-1 Antibody (C-term)

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Purified Rabbit Polyclonal Antibody (Pab)

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

400 µl

Concentration

2 mg/ml

Storage

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

Western blot analysis of Sestrin-1 antibody (C-term) (Cat.#AP7650b) in mouse heart tissue lysates (35ug/lane). Sestrin-1 (arrow) was detected using the purified Pab.

Primary Ab dilution

1:1000

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

"Sestrin-1 Antibody (C-term) (Cat.#AP7650b) western blot analysis in NCI-H460,HL-60 cell line lysates (35ug/lane).This demonstrates the Sestrin-1 antibody detected the Sestrin-1 protein (arrow)."

Primary Ab dilution

1:1000

Flow cytometry

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

Sestrin-1 Antibody (C-term) (Cat. #AP7650b) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Primary Ab dilution

1:10~50